Gpn1 (NM_133756) Mouse Untagged Clone

CAT#: MC211503

Gpn1 (untagged) - Mouse GPN-loop GTPase 1 (Gpn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpn1
• Synonyms: 2410004J02Rik; AI449615; MBDIN; NTPBP; Xab1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211503 representing NM_133756
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCGTGGCTCCATCCGAACCCCAGGCTTCCCGGGCTCCTCAGCCCCCCGTGTGTCTGTTGG
  TGTTGGGAATGGCGGGATCCGGGAAAACTACCTTTGTACAGAGGCTCACAGGACACCTGCACAACAAAGG
  CTGTCCACCGTATGTAATCAACCTGGACCCAGCTGTGCATGAAGTCCCCTTTCCCGCCAATATTGATATC
  CGAGACACAGTGAAATATAAAGAAGTCATGAAGCAATATGGACTTGGGCCAAACGGAGGTATAGTGACCT
  CACTCAACCTCTTTGCTACCAGATTTGATCAGGTGATGAAATTTATTGAGAAGGCCCAGAACACATTCAG
  ATATGTCTTGATTGACACACCTGGACAGATTGAAGTATTTACCTGGTCAGCTTCAGGGACAATCATCACT
  GAGGCCCTGGCTTCCTCATTTCCAACAGTAGTCATCTATGTCATGGATACATCACGGAGCACTAACCCAG
  TGACCTTCATGTCCAATATGCTTTACGCATGCAGCATCTTGTACAAAACCAAGCTGCCTTTCATTGTGGT
  CATGAACAAAACCGACATCATTGACCACAGCTTTGCCGTGGAGTGGATGCAGGATTTTGAGGCTTTCCAG
  GATGCCTTGAACCAAGAGACTACCTACGTCAGTAACCTGACTCGTTCCATGAGCCTGGTGTTGGATGAGT
  TCTACAGCTCACTCAGGGTGGTGGGTGTGTCTGCTGTGGTGGGCACCGGCTTCGATGAACTCTGTACTCA
  GGTCACCAGTGCTGCAGAGGAGTACGAAAGGGAATATCGTCCTGAATACGAGCGTCTGAAGAAATCACTG
  GCAAATGCCCAGAGTAATCAGCAGAAGGAGCAACTGGAGCGTCTTCGGAAGGATATGGGCTCCGTAGCCC
  TGGATCCAGAGGCCGGCAAAGGCAACGCATCTCCTGTGCTGGATCCTTCTGACTTGATCCTGACTCGAGG
  AACCTTGGATGAGGAGGATGAAGAAGCTGACAGTGACACGGATGACATTGACCACAGAGTTACAGAGGAA
  AGCCGTGAAGAGCCAGCATTTCAGAACTTCATGGAGGAGTCAATGGCACACTGGAAGAGGAACAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133756
• Insert Size: 1119 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133756.4, NP_598517.1
• RefSeq Size: 1847 bp
• RefSeq ORF: 1119 bp
• Locus ID: 74254
• UniProt ID: Q8VCE2
• Cytogenetics: 5 B1
• Gene Summary: Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import. Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. May be involved in nuclear localization of XPA.[UniProtKB/Swiss-Prot Function]