Spdya (NM_001142631) Mouse Untagged Clone

CAT#: MC211112

Spdya (untagged) - Mouse speedy homolog A (Xenopus laevis) (Spdya), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spdya
• Synonyms: 4921517J08Rik; 4930548B21Rik; GS4; MLZ-465; Spdy1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211112 representing NM_001142631
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCATAATCAGATGTATTGTGAGACACCACCTACTGTCACTATTCATGTAAAATCAGGCTCAAATA
  GGTCACATCAAACCAGAAAACCTATTAGTCTGAAACGTCCTATTCTTAAAGATAGTTGGGAAGCATCTGA
  AAACAATGCTCAGAATAACAAATCTAAGCGGCCCAGAGGGCCTTGTCTAATCATACAGCGCCAGGAAATG
  ACTGCTTTCTTTAAATTATTTGATGATGATTTAATTCAAGATTTCTTGTGGATGGACTGCTGCTGCAAGA
  TTGCAGACAAGTATCTTTTGGCTATGACCTTTGTTTATTTCAAGAGAGCTAAATTTACTATAAATGAGCA
  TACCAGGATAAATTTCTTTATTGCTCTGTATCTGGCTAATACGGTTGAAGAAGATGAAGAAGAAGCCAAG
  TATGAAATTTTTCCATGGGCTTTAGGGAAAAACTGGAGAAAACTGTTCCCTAATTTCTTAAAGTTAAGGG
  ACCAACTCTGGGACAGAATTGACTATAGGGCTATTGTAAGCAGGCGATGCTGTGAAGAGGTCATGGCCAT
  TGCGCCAACCCATTACATCTGGCAACGAGAGCGGTCTGTGCATCACAGTGGAGCTGTTAGGAACTACAAC
  AGAGATGAGGTTCACCTGCCCAGGGGACCTAGTGCCACACCAGTGGATTGCTCACTGTGTGGTAAAAAAG
  GAAGATACGTGAGACTGGGACTGTCTTCATCCTCATCTTCCTCCAGTGACACAGGAGAGCTAATGGAAAA
  AGATTCTCAGGAACTACACAGTGCATTCTCAGTGGACACGGCAGGTGACCCTCCTCATACCTATTCTCAA
  GGTATGGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142631
• Insert Size: 852 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142631.1, NP_001136103.1
• RefSeq Size: 1247 bp
• RefSeq ORF: 852 bp
• Locus ID: 70891
• UniProt ID: Q5IBH7
• Cytogenetics: 17 E1.3
• Gene Summary: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:15611625). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Interferes with CDKN1B-mediated inhibition of CDK2. Mediates cell survival during the DNA damage process through activation of CDK2 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1.