Apoo (NM_026673) Mouse Untagged Clone

CAT#: MC210715

Apoo (untagged) - Mouse apolipoprotein O (Apoo), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Apoo
• Synonyms: 0610008C08Rik; 1110019O03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210715 representing NM_026673
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAAGGTAATTCAGAGATCTGTGGGGCCAGCCAGCCTGAGCCTGCTCACCTTCAGAGTCTATGCAG
  CACCCAAAAAGGACTCGCCTCACAAAAGTTACATGAAGATCGATGAGCTTTCACTCTACTCAGTTCCTGA
  GGGTCAATCTAAATATGTGGAGGAGCCAAGGACTCAACTTGAAGAAAACATCTCACAACTCCGACATCAT
  TGTGAGCCATATACAAGTTTCTGTCAGGAAATATACTCCCATACTAAACCCAAGGTGGATCACTTTGTCC
  AGTGGGGAGTAGACAACTATAACTATCTTCAAAATGCGCCTCCTGGATTTTTCCCAAGACTCGGTGTTAT
  TGGTTTTGCTGGTTTTGTTGGACTCCTTTTTGCTAGAGGTTCAAAAATAAAGAAGCTGGTGTATCCTCCT
  TTTTTCATGGGATTAGGTGCCTCTGTCTATTACCCACAACAAGCCATCACCATTGCCCAGATCACTGGGG
  AGAAGTTATATGACTGGGGATTACGAGGGTACATAGTTATAGAAGATTTGTGGAAGCAAAATTTTCAGAA
  GCCAGGAAATGTGAAGAATTCACCTGGAAATAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026673
• Insert Size: 597 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026673.4, NP_080949.2
• RefSeq Size: 935 bp
• RefSeq ORF: 597 bp
• Locus ID: 68316
• UniProt ID: Q9DCZ4
• Cytogenetics: X C3
• Gene Summary: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (By similarity). Can induce cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the more frequently occurring transcript. Variant 1 encodes isoform 1.