Fam45a (NM_001167829) Mouse Untagged Clone

CAT#: MC210648

Fam45a (untagged) - Mouse family with sequence similarity 45, member A (Fam45a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fam45a
• Synonyms: 1810055E12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210648 representing NM_001167829
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGTTGTGGCAATGGACACCCAGCTGATGCTCGGAGTCGGGCTGATCGAAAAGGACTCTAATG
  GAGAAGCCCTGTGGGTGTGGTGTTATCCTTCCACGACAGCCTCACTAAGGAATCTGCTGCTCCGAAAATG
  CTGCCTAACTGATGAAAACAAACTTCTCCACCCTTTCGTCTTTGGTCAGTACAGAAGAACATGGTTTTAT
  GTCACGACAGTTGAAGTTCCAGATTCTTCAGTTTTGAAAAAGGTGACTCATTTTTCTATTGTTCTGACCG
  CCAAAGATTTTAACCCAGAGAAATATGCCGCCTTCACTAGGATATTGTGTAGAATATATTTGAAATATGG
  AAGTCCGGTTAAAATGATGGAGAGTTACATTGCAGTTCTCACCAAGGGGATCTGCCAGAGTGAAGAAAAT
  GGCTCTTTCCTTAGTAGAGACTTTGATGGTCGAAAGGCATACCTTGCTGGCTCCATCAAAGACATTGTAT
  CTCAATTTGGAATGGAAACTGTTATTTTACACACAGCATTGATGCTGAAAAAACGAATTGTTGTCTATCA
  CCCCAAAATAGAAGCTGTCCAGGAGTTCACCAGGACTCTGCCTGCCCTGGTGTGGCATCGACAGGATTGG
  ACCATCCTGCATTCTTACATGCACCTTCATGCTGAAGAGCTAGAAGGCCTACAGATGTGCACAGAATTGG
  AGGTGAGCAACAGACCAGACCTCTATGACGTGTTTGTGAATCTGGCAGATAGTGAGATCACCATCGCTCC
  ACTTGCAAAAGAGGCCATGACGATGGGCAAACTGCATAAAGAGATTGGTCAACTAATTGTGCAATCTGCA
  GAAGACCCAGAGAAATCAGACAGCCAGGTTATACAGGACATTGCCCTAAAAACAAAAGAAATCTTCACTC
  ACCTGGCACCATTCTCAGAAGTGTCTGATGATGGGGGAAAAGTCATCCTCAATGTTGAGGCACTGAAGCA
  GCAGCGATTTCCACCCGCAACAGAAAACTTCCTGTATCATCTAGCAGCAGCTGAACAAATGCTGAAGGTC
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167829
• Insert Size: 1053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167829.1, NP_001161301.1
• RefSeq Size: 2345 bp
• RefSeq ORF: 1053 bp
• Locus ID: 67894
• UniProt ID: Q9D8N2
• Cytogenetics: 19 D3
• Gene Summary: Guanine nucleotide exchange factor (GEF) regulating homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion, possibly through activating Rab proteins such as RAB27A and RAB27B. Seems to promote the exchange of GDP to GTP, converting inactive GDP-bound RAB27A and RAB27B into their active GTP-bound form.[UniProtKB/Swiss-Prot Function]