Dusp28 (NM_175118) Mouse Untagged Clone

CAT#: MC210556

Dusp28 (untagged) - Mouse dual specificity phosphatase 28 (Dusp28), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dusp28
• Synonyms: 0710001B24Rik; AV005521
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210556 representing NM_175118
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCTCGGAGGCTGCACCGCCGCCTTTCGCGCGCGTCGCCCCCGCGCTCTTCATCGGGAATGCGC
  GAGCCGCGGGTGCGACGGAGCTGCTGGTGCGCGCGGGCATCACTTTGTGCGTCAATGTCTCCCGCCAGCA
  GCCCGGGCCGCGCGCGCCCGGAGTGGCGGAACTACGCGTACCCGTGTTCGACGACCCAGCTGAGGACCTG
  CTGACACACCTGGAGCCCACCTGTGCCGCCATGGAAGCCGCGGTGCGCGACGGCGGCTCCTGTCTCGTGT
  ACTGCAAGAACGGCCGCAGTCGCTCAGCCGCCGTCTGCACCGCCTACCTAATGCGGCACCGCGGCCACAG
  CCTGGATCGCGCCTTCCAGATGGTGAAGAGCGCCCGCCCGGTAGCCGAGCCCAATTTGGGGTTCTGGGCT
  CAGCTGCAGAAGTACGAGCAGACCCTTCAGGCCCAGGCCATCCTGCCCCGGGAGCCCATTGATCCGGAGT
  AA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_175118
• Insert Size: 492 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175118.3, NP_780327.1
• RefSeq Size: 1491 bp
• RefSeq ORF: 492 bp
• Locus ID: 67446
• UniProt ID: Q8BTR5
• Cytogenetics: 1 D
• Gene Summary: Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate (in vitro). Has almost no detectable activity with phosphotyrosine, even less activity with phosphothreonine and displays complete lack of activity with phosphoserine. The poor activity with phosphotyrosine may be due to steric hindrance by bulky amino acid sidechains that obstruct access to the active site.[UniProtKB/Swiss-Prot Function]