Mrm3 (NM_183263) Mouse Untagged Clone

CAT#: MC210545

Rnmtl1 (untagged) - Mouse RNA methyltransferase like 1 (Rnmtl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mrm3
• Synonyms: 4833420N02Rik; AI255153; HC90; Rnmtl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210545 representing NM_183263
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGCCGGCGAAGGGCATGTGGTGCTCCCTGGGATCATTGCTGCGGGTGGTCCAGACTCGGGACC
  TCAACGCTCGGCGCTGGGTCCGGGCGCTGCGGCGTAGCCCGGTGAGAGTGTTGTCTCCCTCGGGACAGGT
  GGAGGAACGGAAGCGCGCTCCTGACCAGCAGCCTCGCAAGGCGGTCCCTAAGGCCAGTTCCCAGGGACAG
  CGACAGAAACAGCCTCTTGAGACATCCCCATCCCAGACCCCTCACACCTGGGAAGAGGCAGGGCTTCGCT
  ACGATAAGGCCTTTCCCGGGGACAGGAGGCTAAGCAGTGTGATGACAATAGTTAAGTCCAGGCCTTTTCG
  GGAAAAGCAAGGGAAGATCCTACTGGAAGGTCGCAGGCTGATTGCAGATGCTCTCAAGGCTGGAGCTGTG
  CCCAAAGCGTTCTTTTTTAGCCGTCTGGAGTACGTCAAGGAGTTGCCGGTAGACAAGCTGAAAGACGTCA
  GCCTCATTAAGGTGAAATTTGAGGACATCAAGGATTGGTCAGACCTAGTAACGCCACAAGGAATAATGGG
  GATTTTTGCCAAACCTGACCCTGTTAAGATGACATATCCAGAGACTCCGCTTCACCATACACTGCCCCTA
  GTGTTGATTTGTGACAATCTCCGTGACCCTGGGAACCTGGGGACAATCCTGAGATCTGCAGCTGGAGCAG
  GCTGCAGTAAAGTCTTACTCACCAAAGGCTGTGTGGATGCCTGGGAGCCTAAAGTGCTCCGGGCAGGCAT
  GGGGGCACATTTCCAGGTACCCATTGTGAACAATGTGGAATGGGAAACAGTGCCCAATCACTTGCCTCCT
  GACACTCGAGTCTATGTGGCAGACAACTGTGGTCACTATGCCCAGGTTCAGATGTCTGATAAAACCGGTG
  ACCGTGACTGGGCATGTGATCGCCGATTCTTAAAGTTTCACAAGTATGAAGAGGACCTAGACACTAAAAC
  CAGAAAGGACTGGCTGCCCAAACTTGAGGTCCAGAGTTATGATTTGGACTGGACAGGAGCACCAGCAGCT
  GTGGTGATAGGTGGGGAGACGCATGGAGTGAGCCTGGAATCCCTGCAACTGGCCGAGAGTACCGGTGGCA
  AGAGACTGCTGATCCCCGTTGTACCTGGTGTGGACAGTCTGAACTCAGCCATGGCTGCGAGCATCCTGCT
  CTTTGAAGGGAAAAGGCAGCTGAGGATAAAGGTGGAAGACTTGAGCAGGGACAGGAGTTACCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_183263
• Insert Size: 1257 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183263.5, NP_899086.2
• RefSeq Size: 1492 bp
• RefSeq ORF: 1257 bp
• Locus ID: 67390
• UniProt ID: Q5ND52
• Cytogenetics: 11 B5
• Gene Summary: S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1370 (Gm1370) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a conserved modification in the peptidyl transferase domain of the mtLSU rRNA.[UniProtKB/Swiss-Prot Function]