Cenpk (NM_021790) Mouse Untagged Clone

CAT#: MC210203

Cenpk (untagged) - Mouse centromere protein K (Cenpk), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cenpk
• Synonyms: B130045K24Rik; BB232189; C530004N04Rik; Cenp-K; Solt; Solzt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210203 representing NM_021790
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACCGTGTTCTGTCTATTCCAACAACAAAGCTGCATCCTGTACCACTTCTCCGTTGGTAATTTACA
  GTAGTTGCAGTAAGGTGAAGAAAACTATAAAGGATATGTCAGAGAATAAACAAGAAGTACATCCAGATAC
  TATTACAGATGTGGAAGCTGTTATAGATACAGAGGAAGAACTTATTAAAGAATGTGAAGAAATGTGGAAA
  GACATGGAAGATTGTCAGAATAAATTATCACTTATTGGAACTGAGACACTCACGAATGCAGATGCTCAGC
  TCTCATTACTTATTATGCAAATGAAATGTTTGACTGCTGAACTTGGTCAATGGAAGAAAAGAAAACCCGA
  AATAATTCCCCTAAATGAAGACGTTTTGTTAACATTAGGAAAAGAAGAGTTCCAAAAATTGAGATGTGAT
  CTTGAAATGGTACTATCTACCATTCAATCAAAGAATGAAAAGTTAAAAGAAGACTTAGAAAGGGAGCAAC
  AGTGGTTGGATGAACAGCAGCAAATACTGGACACTCTTAACGTATTAAACAGTGATGTGGAAAATCAGGT
  GGTAACATTAACTGAGTCAAGAATCTTTAATGAACTGACAACTAAGATCCGTGGGATAAAGGAATTCAAG
  GAGAAGCTCTTGCTTACCTTGGGTGCTTTTTTAGACAATCATTTTCCTCTGCCTGAAGCAAGTACTCCAA
  AGAAAAGGAAAAACATTCAAGACTCGAATGCACAGCTGATAACACTGAATGAAATATTAGAGATGCTTAT
  AAATAGAATGTTTGATGTTCCACATGATCCATATGTTAAAATTCGTGATTCCTTTTGGCCACCATATATT
  GAGCTACTTCTGCGTTACGGAATTGCTTTGAGACATCCAGAAGATCCATCCCAAATAAGGTTAGAAGCTT
  TCCATCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021790
• Insert Size: 921 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021790.2, NP_068562.1
• RefSeq Size: 1222 bp
• RefSeq ORF: 921 bp
• Locus ID: 60411
• UniProt ID: Q9ESN5
• Cytogenetics: 13 D1
• Gene Summary: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses alternate 5' exon structure, differs in the 5' UTR, and includes an alternate 3' terminal exon compared to variant 2. This transcript also initiates translation at an alternate start codon, resulting in isoform 1, which is longer and has distinct N- and C-termini compared to isoform 2.