Trim54 (NM_021447) Mouse Untagged Clone

CAT#: MC210181

Trim54 (untagged) - Mouse tripartite motif-containing 54 (Trim54), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim54
• Synonyms: 4930486E09Rik; 4930566I02Rik; MURF; MURF-3; MuRF3; Rnf30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210181 representing NM_021447
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTCACGGTGGGTTTCAAGCCGCTGCTAGGGGATGCGCACAACATGGACAACTTGGAGAAGCAGC
  TCATTTGCCCCATCTGCCTGGAGATGTTCTCCAAGCCCGTGGTGATCTTGCCCTGCCAACACAACCTGTG
  CCGCAAGTGTGCCAACGACGTCTTCCAGGCCTCTAATCCTCTGTGGCAATCCCGGGGCTCCACAACGGTG
  TCTTCAGGAGGACGTTTCCGATGCCCATCTTGTAGGCACGAGGTTGTCCTGGACAGGCATGGTGTCTATG
  GCCTGCAGCGGAACCTGCTAGTGGAGAACATCATTGACATCTACAAGCAGGAGTCCTCCCGGCCACTGCA
  CGCCAAGGCTGAACAGCACCTCATGTGTGAGGAGCACGAGGACGAGAAGATCAACATCTACTGCCTGAGC
  TGCGAGGTGCCCACCTGCTCTCTCTGCAAGGTTTTCGGCGCCCACAAGGACTGTGAGGTGGCCCCTCTGC
  CCACCATTTACAAACGCCAGAAGAGTGAGCTGAGCGATGGCATCGCGATGCTGGTGGCGGGCAATGACCG
  TGTGCAGGCAGTGATCACCCAGATGGAGGAGGTGTGCCAGACCATTGAGGACAACAGCCGCAGACAGAAG
  CAACTGTTAAACCAGAGGTTCGAGACCCTGTGCGCGGTTTTGGAGGAGCGCAAGGGCGAACTGCTTCAAG
  CACTGGCCCGGGAGCAGGAGGAGAAGTTGCAGCGCGTGCGGGGCCTCATCCGCCAGTACGGAGACCACTT
  GGAGGGCTCCTCAAAGCTGGTGGAGTCCGCCATCCAGTCCATGGAGGAGCCGCAGATGGCTCTCTACCTC
  CAGCAGGCAAAGGAGCTGATCAACAAGGTCGGGGCAATGTCGAAGGTGGAGCTGGCAGGACGGCCGGAGC
  CAGGCTATGAGAGCATGGAGCAATTCTCTGTGAGCGTGGAGCACGTGGCCGAAATGTTGCGAACCATCGA
  CTTCCAGCCGGGCGCCGCTGGGGATGAAGAGGATGACGACATGGCTTTGGATGGGGAGGAGGGCAATGCG
  GGGCTGGAGGAGGAGCGGCTGGACGTGCCAGAAGGCTCAGGCCTGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021447
• Insert Size: 1101 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021447.2, NP_067422.1
• RefSeq Size: 1548 bp
• RefSeq ORF: 1101 bp
• Locus ID: 58522
• UniProt ID: Q9ERP3
• Cytogenetics: 5 B1
• Gene Summary: May bind and stabilize microtubules during myotubes formation.[UniProtKB/Swiss-Prot Function]