Xrcc2 (NM_020570) Mouse Untagged Clone

CAT#: MC210148

Xrcc2 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 2 (Xrcc2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Xrcc2
• Synonyms: 4921524O04Rik; 8030409M04Rik; RAD51; RecA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210148 representing NM_020570
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTAGCGACTTTCGCAGAGCGGAATCCGGGACAGAGCTCCTTGCCCGACTTGAAGGCAGAAGCTCGT
  TGAAAGAACTAGAACCCAACCTGTTTGCTGATGAAGATTCACCAGTGCACGGTGATATTTTTGAATTTCA
  TGGTCCAGAAGGAACGGGGAAAACAGAAATGCTTTATCATTTAACAGCCCGATGTATACTTCCAAAATCA
  GAGGGTGGACTGCAAATAGAAGTCTTATTTATTGATACAGATTACCACTTTGACATGCTACGGCTCGTGA
  CAGTTCTTGAACACAGACTCTCGCAAAGCTCCGAGGAAGCCATGAAGCTGTGCCTAGCCAGGTTGTTCCT
  GGCGTACTGCAGCAGCAGCATGCAGTTACTGCTCACGCTGCACTCCCTGGAAGCCCTGCTCTGCAGTCGC
  CCCTCTCTCTGCCTTCTCATTGTGGACAGCCTGTCGTCTTTTTACTGGATAGACCGAGTCAGTGGAGGAG
  AGAGTGTGGCCCTGCAGGAGTCAACGCTGCAGAAGTGCTCTCAGCTCCTAGAGCGGCTTGTCACTGAGTA
  CCGCTTGCTGCTTTTTGCGACAACGCAGAGTCTAATGCAGAAAGGCTCAGACTCTGCAGACGGGCCTTCT
  TCCTCCAAGCACCCATGCGATGGAGACATGGGCTACAGAGCCTATCTCTGCAAGGCCTGGCAGAGGGTCG
  TGAAGCACAGGGTCATCTTCTCCAGGGACGACGAGGCCAAGAGCAGCCGGTTCTCATTAGTTTCACGTCA
  TTTAAAAAGTAACAGTTTAAAAAAACACAGTTTTATGGTCAGAGAAAGTGGGGTGGAGTTTTGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020570
• Insert Size: 837 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020570.2, NP_065595.2
• RefSeq Size: 3230 bp
• RefSeq ORF: 837 bp
• Locus ID: 57434
• UniProt ID: Q9CX47
• Cytogenetics: 5 B1
• Gene Summary: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA (By similarity).[UniProtKB/Swiss-Prot Function]