Cdca8 (NM_026560) Mouse Untagged Clone

CAT#: MC209893

Cdca8 (untagged) - Mouse cell division cycle associated 8 (Cdca8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdca8
• Synonyms: 4831429J16Rik; AU044747; BOR; Borealin; D4Ertd421e; DasraB; MESRGP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209893 representing NM_026560
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCCAAGAAACGCAGCAGCCGCGGAACCAGGACCAACACGCTGCGGAGCCGGAAGCTCGCCTCCT
  TCCTGAAGGACTTCGACCGCGAGGTGCAAGTTCGAACCAAGCAAATTGAGTCCGACAGACAGACCCTCCT
  CAAGGAGGTGGAAAATCTGTACAACATCGAGATCCTTCGGCTCCCCAAGGCGCTGCAAGGGATGAAGTGG
  CTTGACTACTTCGCCCTAGGAGGAAACAAGCAGGCCCTGGAAGAGGCAGCAAAAGCTGATCGAGACATCA
  CAGAAATAAACAATTTAACAGCTGAAGCTATTCAGACACCTTTGAAATCTGTTAAAAAGCGAAAGGTAAT
  CGAGGTGGAGGAATCGATAAAGGAAGAAGAAGAAGAGGAAGAAGAAGGAGGAGGAGGAGGAGGAAGAACA
  AAAAAGAGCCATAAGAATCTTCGATCTGCAAAAGTCAAAAGATGCCTTCCATCCAAGAAGAGAACCCAGT
  CCATACAAGGAAGAGGCAGAAGTAAAAGGTTAAGCCATGACTTTGTGACGCCAGCTATGAGCAGGCTGGA
  GCCGTCTCTGGTGAAACCAACCCCAGGCATGACACCTAGGTTTGACTCCCGGGTCTTCAAGACTCCAGGG
  CTACGCACTCCAGCAGCCAAAGAGCAAGTTTACAACATCTCCATCAACGGCAGCCCTCTCGCAGACAGCA
  AAGAGATCTCCCTCAGTGTGCCCATAGGTGGCGGTGCGAGCTTGCGGTTATTGGCCAGTGACTTGCAAAG
  GATTGATATTGCTCAGCTGAATCCAGAGGCTCTGGGAAACATTAGAAAGCTCTCGAGCCGCCTCGCCCAG
  ATCTGCAGCAGCATACGGACGGGCCGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026560
• Insert Size: 870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026560.4, NP_080836.3
• RefSeq Size: 1626 bp
• RefSeq ORF: 870 bp
• Locus ID: 52276
• UniProt ID: Q8BHX3
• Cytogenetics: 4 57.93 cM
• Gene Summary: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment (By similarity).[UniProtKB/Swiss-Prot Function]