Dmrt1 (NM_015826) Mouse Untagged Clone

CAT#: MC209870

Dmrt1 (untagged) - Mouse doublesex and mab-3 related transcription factor 1 (Dmrt1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dmrt1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209870 representing NM_015826
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAACGACGACACATTCGGCAAGCCCTCTACCCCGACGGAGGTTCCTCACGCTCCCGGGGCCCCGC
  CGCAGGGCAAAGCCGGAGGTTACAGCAAAGCTGCCGGGGCGATGGCTGGAGCGGCTGGAGGCTCGGGCGC
  GGGGGGCAGCGGAGGTGCCTCGGGCTCCGGGCCGTCGGGCCTGGGTTCTGGAAGCAAGAAGTCCCCGAGG
  CTGCCCAAATGCGCTCGCTGCAGGAACCACGGCTACGCGTCGCCGCTCAAGGGCCACAAGCGCTTCTGCA
  TGTGGCGGGATTGCCAGTGCAAGAAGTGCAGCCTGATTGCGGAGCGACAGCGGGTGATGGCCGCGCAGGT
  GGCCCTGAGAAGACAGCAGGCCCAGGAAGAAGAACTGGGTATCAGCCACCCAATTCCGCTGCCCAGCGCA
  GCCGAGCTCCTGGTCAAAAGAGAGAATAATGCCAGCAACCCGTGCCTGATGGCCGAGAACAGCAGCTCTG
  CCCAGCCCCCACCGGCCAGCACCCCAACCCCCGCTGCCTCAGAGGGACGCATGGTCATCCAGGATATTCC
  TGCTGTCACCAGCAGAGGGCATATGGAGAACACATCTGACCTGGTATCAGACCCCGCCTACTACAGCAGC
  TTTTACCAGCCTTCTCTGTTTCCTTACTACAACAATCTGTACAACTACCCCCAGTATTCCATGGCCTTGT
  CTGCTGAGTCCTCCTCAGGGGAGGTGGGGAATTCCCTCGGGGGATCCCCCGTGAAGAACAGCCTGCGGAG
  TCTCCCAGCACCTTACGTGCCTGCTCAGACTGGAAACCAGTGGCAGATGAAGACCTCAGAGAGCCGCCAC
  CCAGTGAGCTCCCAGTACCGGATGCACTCATACTACGGGCCTCCCTCCTACCTGGGCCAGAGCATGTCCC
  AGATCTTTACCTTCGAGGAAGGCCCCTCCTACTCAGAAGCCAAAGCCAGTGTGTTCTCGCCCCCCAGCAG
  TCAAGATTCTGGCTTGGTCTCCCTCTCCAGCAGCTCTCCGATGAGCAACGAGAGCTCGAAGGGAGTTCTG
  GAATGTGAGTCTGCGTCCTCGGAACCTAGCAGCTACGCCGTCAACCAGGTCCTGGAAGAGGATGAGGACG
  AGTGAAGCGGACCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_015826
• Insert Size: 1134 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015826.5, NP_056641.2
• RefSeq Size: 2251 bp
• RefSeq ORF: 1125 bp
• Locus ID: 50796
• UniProt ID: Q9QZ59
• Cytogenetics: 19 B
• Gene Summary: Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis.[UniProtKB/Swiss-Prot Function]