Tnnc1 (NM_009393) Mouse Untagged Clone

CAT#: MC209540

Tnnc1 (untagged) - Mouse troponin C, cardiac/slow skeletal (Tnnc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnnc1
• Synonyms: AI874626; cTnC; cTnI; TnC; tncc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209540 representing NM_009393
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGACATCTACAAAGCTGCGGTAGAACAGTTGACAGAGGAGCAGAAGAATGAGTTCAAGGCTGCCT
  TTGATATCTTTGTCCTGGGCGCGGAGGATGGCTGCATCAGCACCAAGGAGCTGGGCAAGGTGATGAGGAT
  GCTGGGCCAGAACCCCACACCTGAGGAGCTGCAGGAGATGATTGACGAAGTAGACGAGGATGGCAGTGGC
  ACAGTGGACTTCGATGAGTTTCTTGTCATGATGGTTCGGTGCATGAAGGACGACAGCAAAGGGAAGTCTG
  AGGAGGAGCTGTCGGATCTCTTCCGCATGTTTGACAAAAACGCTGATGGCTACATTGACTTAGATGAGCT
  GAAGATGATGCTGCAGGCCACAGGTGAGACCATTACGGAAGATGACATTGAAGAGCTCATGAAGGACGGT
  GACAAGAACAACGATGGCCGAATTGACTATGACGAGTTCCTGGAATTCATGAAGGGTGTGGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009393
• Insert Size: 486 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009393.2, NP_033419.1
• RefSeq Size: 703 bp
• RefSeq ORF: 486 bp
• Locus ID: 21924
• UniProt ID: P19123
• Cytogenetics: 14 19.09 cM
• Gene Summary: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.[UniProtKB/Swiss-Prot Function]