Sox7 (NM_011446) Mouse Untagged Clone

CAT#: MC209434

Sox7 (untagged) - Mouse SRY-box containing gene 7 (Sox7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sox7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209434 representing NM_011446
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGCTGCTGGGCGCCTATCCGTGGACCGAGGGACTGGAGTGTCCCGCCCTGGAAGCCGAGCTGT
  CGGATGGGCTGTCGCCGCCCGCCGTCCCCCGACCTTCAGGGGACAAGAGTTCGGAAAGCCGGATCCGGCG
  GCCCATGAATGCCTTCATGGTGTGGGCCAAGGATGAGAGGAAACGTCTGGCAGTGCAGAACCCGGACCTG
  CACAACGCGGAGCTCAGCAAGATGCTGGGAAAGTCATGGAAGGCGCTGACACTGTCCCAGAAGAGACCCT
  ATGTGGATGAGGCAGAGCGGCTGCGCCTGCAGCACATGCAGGATTACCCCAACTACAAGTACCGGCCCCG
  CAGGAAGAAACAAGGCAAGCGCCTCTGCAAGCGCGTGGACCCTGGCTTCCTCCTCAGCTCCCTCTCTCGT
  GACCAGAACACGCTGCCTGAGAAAAACGGCATTGGCAGGGGGGAGAAGGAGGACAGGGGTGAGTACTCCC
  CAGGGGCCACCTTGCCTGGACTGCACAGCTGCTACCGCGAAGGTGCAGCTGCTGCCCCTGGCAGTGTGGA
  CACGTATCCCTACGGGCTGCCCACACCTCCGGAGATGTCGCCCCTGGATGCGCTGGAGCCAGAGCAGACC
  TTCTTCTCGTCCTCATGTCAGGAGGAGCATGGTCACCCCCATCACCTCCCCCATCTACCAGGGCCCCCTT
  ACTCACCGGAGTTCACACCTAGTCCCCTCCACTGCAGCCACCCTCTAGGTTCTTTAGCCCTTGGCCAATC
  CCCAGGGGTTTCTATGATGTCCTCTGTTTCTGGATGTCCCCCATCTCCAGCCTATTACTCCCATGCCACC
  TACCACCCTCTCCACCCCAACCTCCAGGCCCACCTGGGCCAGCTGTCCCCACCTCCGGAGCACCCTGGCT
  TTGACACCTTGGATCAGCTAAGCCAGGTGGAACTTCTGGGAGACATGGATCGCAATGAATTTGATCAGTA
  TTTGAACACTCCCGGCCACCCTGACTCTGCTGCAGGGGTTGGAACCCTCACTGGGCATGTCCCGCTCTCC
  CAGGGGACTCCCACAGGCCCTACAGAGACCAGCCTCATCTCAGTCCTGGCTGATGCCACGGCCACGTATT
  ACAACAGCTACAGTGTGTCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011446
• Insert Size: 1143 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011446.1, NP_035576.1
• RefSeq Size: 3266 bp
• RefSeq ORF: 1143 bp
• Locus ID: 20680
• UniProt ID: P40646
• Cytogenetics: 14 D1
• Gene Summary: Binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. May be required for the survival of both hematopoietic and endothelial precursors during specification. May play a role in skeletal myogenesis and up-regulate the expression of muscle markers, such as PAX3/PAX7 and Meox1. Competes with GATA4 for binding and activation of the FGF3 promoter. Represses Wnt/beta-catenin-stimulated transcription. Probably acts by targeting CTNNB1 to proteasomal degradation. Binds the DNA sequence 5'-AACAAT-3'.[UniProtKB/Swiss-Prot Function]