Ptcra (NM_011195) Mouse Untagged Clone

CAT#: MC209251

Ptcra (untagged) - Mouse pre T-cell antigen receptor alpha (Ptcra), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptcra
• Synonyms: gp33; pT-alpha; pT-alpha-TCR; pTa; pTalpha; pT[a]
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209251 representing NM_011195
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCTCCACGAGTGGGCCATGGCTAGGACATGGCTGCTGCTGCTTCTGGGCGTCAGGTGTCAGGCTC
  TACCATCAGGCATCGCTGGCACCCCCTTTCCGTCTCTGGCTCCACCCATCACACTGCTGGTAGATGGAAG
  GCAGCACATGCTGGTGGTTTGCCTGGTCCTCGATGCGGCACCCCCTGGCCTTGACAACCCTGTCTGGTTC
  TCAGCTGGCAATGGCAGTGCCCTAGACGCCTTCACCTATGGGCCCTCTCTGGCACCGGACGGCACCTGGA
  CTAGCCTGGCCCAGCTCTCCTTGCCTTCTGAAGAGCTGGAAGCCTGGGAGCCCTTGGTCTGTCACACCAG
  GCCTGGGGCTGGGGGACAGAACCGGAGCACACACCCCCTCCAGCTGTCAGGGGAATCTTCGACAGCCAGG
  AGCTGCTTTCCGGAGCCTCTCGGGGGGACACAGCGGCAGGTACTGTGGCTGAGCCTACTGCGCCTGCTCC
  TCTTCAAACTGCTTCTGCTCGATGTGCTCCTGACCTGCAGCCACCTCCGCCTCCATGTGCTGGCGGGCCA
  GCACCTCCAGCCACCACCCTCACGCAAGTCCCTGCCTCCCACCCACAGAATTTGGACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011195
• Insert Size: 621 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011195.2, NP_035325.1
• RefSeq Size: 1283 bp
• RefSeq ORF: 621 bp
• Locus ID: 19208
• UniProt ID: P0C6B2
• Cytogenetics: 17 C
• Gene Summary: The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. Isoform 1 acts to retain most TCRB intracellularly, while isoform 2 permits higher levels of cell surface TCRB expression and facilitates signaling from the CD3-TCRB complex.[UniProtKB/Swiss-Prot Function]