Endou (NM_001168693) Mouse Untagged Clone

CAT#: MC209208

Endou (untagged) - Mouse endonuclease, polyU-specific (Endou), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Endou
• Synonyms: Pp11r; Tcl-30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209208 representing NM_001168693
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCCCGTGTTCCTTTTATAGTGGCTATGCTCTGTGGTCTAGCCTGGGCTGGAAACCTGGAGTCGT
  GCGCATCTCGCTGTCATGAGAAATTTAACCGGGATGCTGTCTGCCAGTGTGATCGCCGGTGTCCCCAGCA
  TGATGACTGCTGTGACGACTATGAACATCTGTGCACTGCTGAAGAAGGACCCCCAGAGCCAGAGGCATTC
  CTGGATCCAGAGGACAAGATCCCAACCAGCCACCTCTACTCCGCACCGAGCTCCTGCCAGGGCCGCTGCC
  GGGAAGCCTACGACAAGCACCACCCTTGCCACTGTAATGACCGCTGCCCAGAGTTTGGAAACTGCTGTGA
  GGATTTTGACAGTCTGTGTGGTGGTGATCATGAGGGTTTCTCCCACAGCAGCGATGCCGTCACTAAGGAG
  GAGCTTGAGAGCATCTCCGAGACCATCTACAGGGTAGATACCAACAAAGCCCAGAAGGAAGACATTGTGC
  TCAACAGTCAGAACCGGATCTCCCCATCAGAGACGGGGAACCAAGTGGATCGAAGCCCAGAGCCGCTCTT
  CACTTACGTCAACGAGAAGCTGTTCTCCAAGCCTACCTATGCAGCCTTTATCAACTTACTCAACAATTAC
  CAGCGGGCCACAGGCCACGGGGAGCATTTCAGTGCCCAGCAACTGGAGGAGCAGGGTGTCTTCCTCAGGG
  AGGTCATGAAGACGGCTGTCATGAAGGAACTCTATAGCTTTCTCCATCACCAAAACCGTTACAGCTCAGA
  ACAAGAGTTTGTGGACGATTTGAAGAACATGTGGTTTGGGCTGTACTCGAGAGGCAATGATGAAGGGGAC
  TCGAGTGGCTTTGAACATGTCTTCTCTGGCGAAGTGAAGAAAGGCAAGGTCACTGGCTTTCATAACTGGA
  TCCGCTTCTACCTGCAGGAGAAGGAGGGTCTGCTGGACTACTACAGTCACAACTACGACGGGCCCTGGGA
  CTCCTACCCCGATGTCCTGGCCATGCAGTTCAATTGGGATGGCTACTACAAAGAAGTGGGCTCGGTCTTC
  ATTGGCAGCAGCCCTGAGTTCGAGTTTGCCCTTTACTCTCTGTGCTTCATCACCAGGCCAGGCAAAAAGT
  GCCATCTGAGCCTGGGTGGCTATCCTCTGGCCATCCAGACCTACACCTGGGATAAGACCACCTACGGAAA
  TGGCAAGAAGTACATTGCCACAGCCTACGTGGTGTCTTCTTCCCAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001168693
• Insert Size: 1239 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168693.1, NP_001162164.1
• RefSeq Size: 2405 bp
• RefSeq ORF: 1239 bp
• Locus ID: 19011
• UniProt ID: Q3V188
• Cytogenetics: 15 53.7 cM
• Gene Summary: Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains different segments for its 5' UTR and coding region, compared to variant 1. The predicted protein (isoform 2) results from translation initiation at an upstream AUG and has a shorter N-terminus, when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.