Msi1 (NM_008629) Mouse Untagged Clone

CAT#: MC208979

Msi1 (untagged) - Mouse Musashi homolog 1(Drosophila) (Msi1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Msi1
• Synonyms: m-Msi-1; Msi1h; Musahi1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208979 representing NM_008629
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACTGACGCGCCCCAGCCCGGCCTCGCCTCCCCGGACTCGCCGCACGACCCCTGCAAGATGTTCA
  TCGGAGGACTCAGTTGGCAGACCACGCAGGAAGGGCTGCGCGAATACTTCGGCCAGTTCGGGGAGGTGAA
  AGAGTGTCTGGTGATGCGGGACCCCCTGACCAAAAGATCCAGGGGTTTCGGCTTCGTCACTTTCATGGAC
  CAGGCGGGGGTGGATAAAGTGCTGGCGCAATCGCGGCACGAGCTCGACTCCAAAACAATTGACCCCAAGG
  TGGCCTTTCCTCGAAGAGCACAGCCTAAGATGGTCACTCGGACGAAGAAGATCTTCGTGGGGGGGCTGTC
  TGTGAACACCACGGTGGAAGATGTGAAACACTATTTCGAGCAGTTCGGAAAGGTGGATGATGCCATGCTG
  ATGTTCGACAAAACCACCAACAGGCACAGAGGGTTTGGATTTGTCACGTTTGAGAGCGAGGACATCGTAG
  AGAAAGTTTGTGAGATCCACTTCCATGAAATCAACAACAAAATGGTGGAATGCAAGAAAGCCCAGCCAAA
  GGAGGTGATGTCCCCGACAGGCTCAGCCCGGGGCAGGTCTCGGGTCATGCCCTACGGAATGGATGCCTTC
  ATGCTGGGTATTGGGATGCTGGGTTACCCAGGGTTCCAAGCCACGACCTACGCCAGCCGGAGTTACACAG
  GCCTTGCCCCTGGTTACACCTACCAGTTCCCCGAATTCCGTGTAGAGCGGAGCCCTCTCCCGAGCGCCCC
  AGTCCTCCCCGAGCTCACAGCTATCCCTCTCACGGCTTATGGGCCCATGGCGGCGGCAGCGGCGGCGGCA
  GCTGTAGTTCGAGGGACAGGCTCTCACCCCTGGACGATGGCTCCCCCTCCAGGTTCCACTCCCAGCCGCA
  CAGGGGGCTTCCTAGGGACCACAAGCCCCGGCCCCATGGCTGAGCTCTACGGGGCAGCCAACCAGGACTC
  CGGGGTCAGCAGTTACATCAGCGCCGCCAGCCCCGCCCCCAGCACTGGTTTCGGCCACAGTCTTGGGGGT
  CCCTTGATTGCCACAGCCTTCACCAATGGGTACCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008629
• Insert Size: 1089 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008629.1, NP_032655.1
• RefSeq Size: 1551 bp
• RefSeq ORF: 1089 bp
• Locus ID: 17690
• UniProt ID: Q61474
• Cytogenetics: 5 F
• Gene Summary: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.[UniProtKB/Swiss-Prot Function]