Mitf (NM_008601) Mouse Untagged Clone

CAT#: MC208963

Mitf (untagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mitf
• Synonyms: BCC2; Bhlhe32; bw; Gsfbcc2; mi; vit; Vitiligo; Wh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208963 representing NM_008601
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAAATGCTAGAATACAGTCACTACCAGGTGCAGACCCACCTGGAAAACCCCACCAAGTACCACA
  TACAGCAAGCTCAGAGGCACCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATGCCAGCCA
  AGTCCTGAGCTCACCATGTCCAAACCAGCCTGGCGACCATGCCATGCCACCAGTGCCGGGGAGCAGCGCA
  CCCAACAGCCCTATGGCTATGCTCACTCTTAACTCCAACTGTGAAAAAGAGGCATTTTATAAGTTTGAGG
  AGCAGAGCAGGGCAGAGAGTGAGTGCCCAGGTATGAACACGCACTCTCGAGCGTCGTGCATGCAGATGGA
  TGATGTAATTGATGACATCATCAGCCTGGAATCAAGTTATAATGAAGAAATTTTGGGCTTGATGGATCCG
  GCCTTGCAAATGGCAAATACGTTACCCGTCTCTGGAAACTTGATCGACCTCTACAGCAACCAGGGCCTGC
  CACCGCCAGGCCTTACCATCAGCAACTCCTGTCCAGCCAACCTTCCCAACATAAAAAGGGAGCTCACAGC
  GTGTATTTTCCCCACAGAGTCTGAAGCAAGAGCATTGGCTAAAGAGAGGCAGAAAAAGGACAATCACAAC
  TTGATTGAACGAAGAAGAAGATTTAACATAAACGACCGCATTAAGGAGCTAGGTACTCTGATCCCCAAGT
  CAAATGATCCAGACATGCGGTGGAACAAGGGAACCATTCTCAAGGCCTCTGTGGACTACATCCGGAAGTT
  GCAACGGGAACAGCAACGAGCTAAGGACCTTGAAAACCGACAGAAGAAGCTGGAGCATGCGAACCGGCAC
  CTGCTGCTCAGAGTACAGGAGCTGGAGATGCAGGCTAGAGCGCATGGACTTTCCCTTATCCCATCCACCG
  GTCTCTGCTCGCCTGATCTGGTGAATCGGATCATCAAGCAAGAACCAGTTCTTGAGAACTGCAGCCAGGA
  ACTTGTACAGCACCAGGCAGACCTGACATGTACGACAACTCTGGATCTCACGGACGGTACCATCACCTTT
  ACCAACAACCTCGGCACCATGCCGGAGAGCAGCCCGGCCTACAGCATCCCCAGGAAGATGGGCTCCAACT
  TGGAAGACATCCTGATGGACGATGCCCTCTCACCTGTTGGAGTCACCGACCCACTGCTGTCATCAGTGTC
  GCCAGGAGCTTCAAAAACAAGCAGCCGGAGGAGCAGTATGAGCGCAGAAGAAACGGAGCATGCGTGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008601
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008601.3, NP_032627.1
• RefSeq Size: 4581 bp
• RefSeq ORF: 1260 bp
• Locus ID: 17342
• UniProt ID: Q08874
• Cytogenetics: 6 45.05 cM
• Gene Summary: This transcription factor serves at a critical point between extracellular signaling and downstream targets in cell specification in early eye and neural crest development. Mutant alleles have been identified that generate distinct phenotypes. Some of these alleles are being used to model the human diseases Waardenburg syndrome IIa and Tietz syndrome. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2, also known as Mitf-M) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by {transcript alignments.