Mesp1 (NM_008588) Mouse Untagged Clone

CAT#: MC208959

Mesp1 (untagged) - Mouse mesoderm posterior 1 (Mesp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mesp1
• Synonyms: bHLHc5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208959 representing NM_008588
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCCCCTGTGCGAGCCGCGCTCCGAGTCCTGGATCCTGAGTCCCGCTGGTCGGCAGCCACCGA
  TGCCTTCCGATGGGAACAGCGTCTGCTCCCCAGCCTGGTCCTCGGACCCGTGGGACGGTGCCCAGGCCAG
  CAGCCCTGCACCACCCTGCGCCCGCCCGGCCCGGCGTGCTGGGACCCCGGGTAGGCGCGGGACGCACGGT
  AGCCGCCTGGGTAGCGGACAGCGGCAGAGCGCCAGCGAGCGGGAGAAGCTACGTATGCGCACACTCGCCC
  GCGCGCTGCACGAGCTGCGCCGCTTCTTGCCGCCATCCGTGGCACCAACCGGCCAGAACCTGACCAAGAT
  CGAGACGCTGCGCCTGGCCATCCGCTACATTGGCCACCTGTCGGCTGTGCTGGGACTCAGCGAGGACAAC
  CTCCGGCGACAGCGGCACGCGGTGTCACCTCGAGGCTGCCCGCTGTGCCCCGACAGCGACCTGGCGCAGT
  CGCAGTCGCTCGGTCCCCGTTTAAGCCCGGCCGTCTGCAGCGGGGTGTCGTGGGGATCCCCGCCTGCCTA
  CCCTAGACCCCGAGTCGCCGCAGAATCGTGGGACCCATCGTTCCTGTACGCAGAAACAGCATCCCAGGAA
  AGGCAGGAAATGGAGCCCAGTCCCTCATCTCCGCTCTTCAGCAGCGACATGCTGGCTCTTCTAGAAACCT
  GGACGCCGCCGCAGGAGTGGCCGCCTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008588
• Insert Size: 732 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008588.2, NP_032614.2
• RefSeq Size: 1098 bp
• RefSeq ORF: 732 bp
• Locus ID: 17292
• UniProt ID: P97309
• Cytogenetics: 7 45.16 cM
• Gene Summary: Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.[UniProtKB/Swiss-Prot Function]