Eda (NM_001177941) Mouse Untagged Clone

CAT#: MC208413

Eda (untagged) - Mouse ectodysplasin-A (Eda), transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eda
• Synonyms: Ed1; Eda-A1; Eda-A2; EDA1; HED; Ta; tabby; Tnlg7c; XLHED
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208413 representing NM_001177941
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTACCCAGAGGTAGAGCGCAGGGAACCCCTGCCTGCGGCAGCGCCAAGGGAGCGGGGCAGCCAGG
  GCTGCGGCTGTCGCGGGGCCCCTGCTCGGGCGGGCGAAGGGAACAGCTGCCGGCTCTTCCTGGGTTTCTT
  TGGCCTCTCGCTGGCCCTCCACCTGCTGACGCTGTGCTGCTACCTAGAGTTGCGGTCCGAATTGCGGCGG
  GAACGGGGAACCGAGTCCCGCCTCGGTGGCCCGGGTGCTCCTGGCACCTCTGGCACCCTAAGCAGCCCTG
  GGAGCCTCGACCCGGTGGGTCCCATCACCCGCCACCTGGGGCAGCCGTCCTTTCAACAGCAGCCTTTGGA
  ACCGGGAGAAGATCCACTCCCCCCTGACTCCCAGGACCGGCACCAGATGGCCCTCCTGAATTTCTTCTTT
  CCTGATGAAAAGGCATATTCTGAAGAGGAAAGTAGGCGTGTTCGCCGCAATAAGAGAAGCAAAAGTGGTG
  AAGGAGCAGATGGAAAGAAGGCAGGGCCACCTGGGCCCAACGGCCCCCCAGGACCTCCAGGACCTCCGGG
  ACCCCAGGGACCTCCAGGGATTCCAGGAATTCCTGGGATTCCAGGAACAACTGTTATGGGACCACCTGGC
  CCACCTGGCCCTCCTGGTCCTCAAGGACCCCCTGGCCTCCAAGGACCTTCTGGTGCTGCTGATAAAACTG
  GAACTCGGGAAAATCAGCCAGCTGTGGTGCATCTGCAGGGCCAAGGGTCAGCAATTCAAGTCAAAAATGG
  TGGAGTGCTCAATGACTGGTCTCGCATCACTATGAACCCTAAGGTGTTTAAACTACATCCCCGCAGCGGG
  GAGCTGGAGGTACTGGTGGACGGCACCTACTTCATCTATAGTCAGGTCTACTACATCAACTTCACTGACT
  TTGCCAGCTACGAGGTGGTGGTGGATGAGAAGCCCTTCCTGCAGTGCACCCGCAGCATTGAGACAGGGAA
  GACCAACTACAACACTTGCTATACTGCAGGCGTGTGCCTCCTCAAGGCCAGGCAGAAAATCGCCGTGAAG
  ATGGTGCACGCTGACATCTCTATCAATATGAGCAAGCACACCACCTTCTTCGGGGCCATCAGGCTGGGCG
  AAGCCCCTGCATCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177941
• Insert Size: 1137 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177941.1, NP_001171412.1
• RefSeq Size: 4916 bp
• RefSeq ORF: 1137 bp
• Locus ID: 13607
• Cytogenetics: X 43.59 cM
• Gene Summary: Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R (By similarity). May also play a role in cell adhesion (PubMed:10534613).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) lacks an in-frame exon and two in-frame small segments in the CDS, as compared to variant 1. The resulting isoform (6) is shorter but has the same N- and C-termini, as compared to isoform 1.