Eda (NM_010099) Mouse Untagged Clone

CAT#: MC208411

Eda (untagged) - Mouse ectodysplasin-A (Eda), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eda
• Synonyms: Ed1; Eda-A1; Eda-A2; EDA1; HED; Ta; tabby; Tnlg7c; XLHED
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208411 representing NM_010099
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTACCCAGAGGTAGAGCGCAGGGAACCCCTGCCTGCGGCAGCGCCAAGGGAGCGGGGCAGCCAGG
  GCTGCGGCTGTCGCGGGGCCCCTGCTCGGGCGGGCGAAGGGAACAGCTGCCGGCTCTTCCTGGGTTTCTT
  TGGCCTCTCGCTGGCCCTCCACCTGCTGACGCTGTGCTGCTACCTAGAGTTGCGGTCCGAATTGCGGCGG
  GAACGGGGAACCGAGTCCCGCCTCGGTGGCCCGGGTGCTCCTGGCACCTCTGGCACCCTAAGCAGCCCTG
  GGAGCCTCGACCCGGTGGGTCCCATCACCCGCCACCTGGGGCAGCCGTCCTTTCAACAGCAGCCTTTGGA
  ACCGGGAGAAGATCCACTCCCCCCTGACTCCCAGGACCGGCACCAGATGGCCCTCCTGAATTTCTTCTTT
  CCTGATGAAAAGGCATATTCTGAAGAGGAAAGTAGGCGTGTTCGCCGCAATAAGAGAAGCAAAAGTGGTG
  AAGGAGCAGATGGTCCTGTTAAAAACAAGAAAAAGGGAAAGAAGGCAGGGCCACCTGGGCCCAACGGCCC
  CCCAGGACCTCCAGGACCTCCGGGACCCCAGGGACCTCCAGGGATTCCAGGAATTCCTGGGATTCCAGGA
  ACAACTGTTATGGGACCACCTGGCCCACCTGGCCCTCCTGGTCCTCAAGGACCCCCTGGCCTCCAAGGAC
  CTTCTGGTGCTGCTGATAAAACTGGAACTCGGGAAAATCAGCCAGCTGTGGTGCATCTGCAGGGCCAAGG
  GTCAGCAATTCAAGTCAAAAATGATCTTTCAGGTGGAGTGCTCAATGACTGGTCTCGCATCACTATGAAC
  CCTAAGGTGTTTAAACTACATCCCCGCAGCGGGGAGCTGGAGGTACTGGTGGACGGCACCTACTTCATCT
  ATAGTCAGGTAGAAGTCTACTACATCAACTTCACTGACTTTGCCAGCTACGAGGTGGTGGTGGATGAGAA
  GCCCTTCCTGCAGTGCACCCGCAGCATTGAGACAGGGAAGACCAACTACAACACTTGCTATACTGCAGGC
  GTGTGCCTCCTCAAGGCCAGGCAGAAAATCGCCGTGAAGATGGTGCACGCTGACATCTCTATCAATATGA
  GCAAGCACACCACCTTCTTCGGGGCCATCAGGCTGGGCGAAGCCCCTGCATCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010099
• Insert Size: 1176 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010099.2, NP_034229.1
• RefSeq Size: 4955 bp
• RefSeq ORF: 1176 bp
• Locus ID: 13607
• UniProt ID: O54693
• Cytogenetics: X 43.59 cM
• Gene Summary: Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R (By similarity). May also play a role in cell adhesion (PubMed:10534613).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.