Eda (NM_010099) Mouse Tagged ORF Clone

CAT#: MG224097

Eda (tGFP-tagged) - Mouse ectodysplasin-A (Eda) transcript variant 1, (10ug)

Product Images

Circular map for MG224097

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eda
• Synonyms: Ed1; Eda-A1; Eda-A2; EDA1; HED; Ta; tabby; Tnlg7c; XLHED
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224097 representing NM_010099
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTACCCAGAGGTAGAGCGCAGGGAACCCCTGCCTGCGGCAGCGCCAAGGGAGCGGGGCAGCCAGG
  GCTGCGGCTGTCGCGGGGCCCCTGCTCGGGCGGGCGAAGGGAACAGCTGCCGGCTCTTCCTGGGTTTCTT
  TGGCCTCTCGCTGGCCCTCCACCTGCTGACGCTGTGCTGCTACCTAGAGTTGCGGTCCGAATTGCGGCGG
  GAACGGGGAACCGAGTCCCGCCTCGGTGGCCCGGGTGCTCCTGGCACCTCTGGCACCCTAAGCAGCCCTG
  GGAGCCTCGACCCGGTGGGTCCCATCACCCGCCACCTGGGGCAGCCGTCCTTTCAACAGCAGCCTTTGGA
  ACCGGGAGAAGATCCACTCCCCCCTGACTCCCAGGACCGGCACCAGATGGCCCTCCTGAATTTCTTCTTT
  CCTGATGAAAAGGCATATTCTGAAGAGGAAAGTAGGCGTGTTCGCCGCAATAAGAGAAGCAAAAGTGGTG
  AAGGAGCAGATGGTCCTGTTAAAAACAAGAAAAAGGGAAAGAAGGCAGGGCCACCTGGGCCCAACGGCCC
  CCCAGGACCTCCAGGACCTCCGGGACCCCAGGGACCTCCAGGGATTCCAGGAATTCCTGGGATTCCAGGA
  ACAACTGTTATGGGACCACCTGGCCCACCTGGCCCTCCTGGTCCTCAAGGACCCCCTGGCCTCCAAGGAC
  CTTCTGGTGCTGCTGATAAAACTGGAACTCGGGAAAATCAGCCAGCTGTGGTGCATCTGCAGGGCCAAGG
  GTCAGCAATTCAAGTCAAAAATGATCTTTCAGGTGGAGTGCTCAATGACTGGTCTCGCATCACTATGAAC
  CCTAAGGTGTTTAAACTACATCCCCGCAGCGGGGAGCTGGAGGTACTGGTGGACGGCACCTACTTCATCT
  ATAGTCAGGTCTACTACATCAACTTCACTGACTTTGCCAGCTACGAGGTGGTGGTGGATGAGAAGCCCTT
  CCTGCAGTGCACCCGCAGCATTGAGACAGGGAAGACCAACTACAACACTTGCTATACTGCAGGCGTGTGC
  CTCCTCAAGGCCAGGCAGAAAATCGCCGTGAAGATGGTGCACGCTGACATCTCTATCAATATGAGCAAGC
  ACACCACCTTCTTCGGGGCCATCAGGCTGGGCGAAGCCCCTGCATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224097 representing NM_010099
  Red=Cloning site Green=Tags(s)
  
  MGYPEVERREPLPAAAPRERGSQGCGCRGAPARAGEGNSCRLFLGFFGLSLALHLLTLCCYLELRSELRR
  ERGTESRLGGPGAPGTSGTLSSPGSLDPVGPITRHLGQPSFQQQPLEPGEDPLPPDSQDRHQMALLNFFF
  PDEKAYSEEESRRVRRNKRSKSGEGADGPVKNKKKGKKAGPPGPNGPPGPPGPPGPQGPPGIPGIPGIPG
  TTVMGPPGPPGPPGPQGPPGLQGPSGAADKTGTRENQPAVVHLQGQGSAIQVKNDLSGGVLNDWSRITMN
  PKVFKLHPRSGELEVLVDGTYFIYSQVYYINFTDFASYEVVVDEKPFLQCTRSIETGKTNYNTCYTAGVC
  LLKARQKIAVKMVHADISINMSKHTTFFGAIRLGEAPAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010099
• ORF Size: 1167 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010099.2, NP_034229.1
• RefSeq Size: 4955 bp
• RefSeq ORF: 1176 bp
• Locus ID: 13607
• UniProt ID: O54693
• Cytogenetics: X 43.59 cM
• Gene Summary: Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R (By similarity). May also play a role in cell adhesion (PubMed:10534613).[UniProtKB/Swiss-Prot Function]