Atp5f1 (NM_009725) Mouse Untagged Clone

CAT#: MC208165

Atp5f1 (untagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 (Atp5f1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp5f1
• Synonyms: C76477
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208165 representing NM_009725
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCCGGGTGGTGCTTTCTGCTGCCGCCACAGCGGCCCCGTGTCTGAAGAACGCGGCCGCCCTAG
  GTCCAGGGGTATTACAGGCAACAAGGGCCTTTCACACAGGACAGCCTCGCCTTGCCCCTCTACCACCTCT
  TCCTGAATATGGAGGAAAAGTGCGTCTTGGGCTGATTCCTGAGGAATTTTTCCAGTTCCTTTACCCTAAG
  ACTGGTGTAACAGGACCTTATGTGCTTGGAACTGGACTTAGCTTGTATTTTCTATCCAAAGAAATATATG
  TGATTACCCCAGAGACCTTCTCTACCATATCAGTAGTAGGGTTGATAGTCTATGTGATTAAGAAATATGG
  CGCCTCTTTTGGAGAATTTATTGACAAACTTAATGAGGAAAAAATTGCTCAACTAGAAGAAGTAAAGCAG
  TCGAGCATGAAACAAATCCAGGATGCAATCGACATGGAGAAGGCACAGCAGGCACTGGTTCAGAAGCGCC
  ATTACCTCTTCGATGTGCAGAGGAATAACATTGCCCTGGCCTTGGAGGTCACTTACCGGGAACGGCTACA
  TAAAGCATATAAGGAGGTAAAGAATCGCCTGGACTACCACATCTCTGTACAGAACATGATGCGTCGCAAG
  GAGGAAGAACACATGATAGACTGGGTAGAAAAGCATGTGGTGAAGAGCATTTCTGTACAGCAGGAAAAGG
  AGACCATTGCCAAGTGCATTGAAGATCTAAAGCTGCTTGCAAAGAAGGCTCAAGCTCAGCCAATTATGTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009725
• Insert Size: 771 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009725.4, NP_033855.2
• RefSeq Size: 1641 bp
• RefSeq ORF: 771 bp
• Locus ID: 11950
• UniProt ID: Q9CQQ7
• Cytogenetics: 3 46.46 cM
• Gene Summary: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).