Tifa (NM_145133) Mouse Untagged Clone

CAT#: MC207861

Tifa (untagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tifa
• Synonyms: T2bp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207861 representing NM_145133
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCTTTGAAGACGCTGATACAGAGGAGACGGTCACTTGTCTCCAGATGACCATTTACCATCCTG
  GCCAACAAAGTGGGATATTTAAATCAATAAGGTTTTGCAGCAAAGAGAAGTTTCCTTCCATTGAAGTGGT
  GAAATTTGGACGCAATTCCAACATGTGCCAGTATACGTTTCAGGACAAACAGGTGTCCCGAATTCAGTTT
  GTTTTACAGCCGTTTAAACAGTTCAACAGCTCCGTTCTCTCGTTTGAAATAAAAAACATGAGCAAGAAAA
  CCAGTTTGATGGTAGACAACCAGGAGCTCGGCTACCTCAATAAAATGGACCTGCCTTACAAGTGTATGCT
  CAGGTTCGGAGAGTATCAGTTCCTGTTGCAGAAGGAAGACGGAGAGTCGGTGGAATCTTTTGAGACTCAA
  TTTATCATGTCTTCAAGACCTCTCTTGCAAGAAAACAACTGGCCAACACAGAATCCCATACCAGAGGATG
  GGATGTATTCTTCATACTTCACCCACAGAAGTTCTCCTTCAGAAATGGATGAAAACGAACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145133
• Insert Size: 555 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145133.3, NP_660115.1
• RefSeq Size: 2164 bp
• RefSeq ORF: 555 bp
• Locus ID: 211550
• UniProt ID: Q793I8
• Cytogenetics: 3 G2
• Gene Summary: Adapter molecule that plays a key role in the activation of proinflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs) (PubMed:11798190). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism (By similarity). TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of proinflammatory NF-kappa-B signaling (By similarity).[UniProtKB/Swiss-Prot Function]