Chrdl1 (NM_031258) Mouse Untagged Clone

CAT#: MC207786

Chrdl1 (untagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chrdl1
• Synonyms: CHL; CHL1; Nrln1; VOPT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207786 representing NM_031258
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCATGAAATACATCATTTCCTTATTTTTCATCTTTGTTTTCCTAGAAGGAAGCAAAACAGAAC
  AAGTAAAACACTCAGACACATATTGCGTGTTTCAAGACAAGAAGTATAGAGTGGGTGAGAAATGGCATCC
  CTACCTGGAACCGTATGGACTGGTTTACTGTGTGAACTGCATCTGCTCTGAGAATGGGAATGTGCTTTGC
  AGCCGAGTCAGATGTCCAAGTCTTCATTGCCTTTCACCCGTGCATATTCCTCATCTCTGTTGCCCCCGCT
  GCCCAGACTCCTTACCACCAGTGAACAATAAGGTGACCAGCAAGTCATGCGAATACAATGGAACCACTTA
  CCAACATGGAGAACTGTTCATAGCTGAAGGGCTCTTTCAGAACCGGCAACCCAATCAGTGCAGTCAGTGT
  AGCTGCTCGGAGGGGAATGTATACTGTGGTCTCAAGACTTGCCCCAAACTGACCTGTGCATTCCCAGTCT
  CTGTTCCAGATTCTTGCTGCCGAGTATGCAGAGGGGATGCAGAATTATCGTGGGAACATGCGGATGGTGA
  TATCTTCCGGCAACCTGCCAACAGAGAAGCAAGACATTCTTACCTCCGTTCCCCCTACGATCCTCCACCA
  AACAGACAAGCTGGAGGTCTTCCCCGCTTTCCTGGGAGCAGAAGTCACCGGGGAGCTGTTATAGATTCCC
  AGCAAGCATCCGGGACCATCGTGCAGATTGTCATCAATAACAAGCACAAACATGGACAAGTGTGTGTTTC
  CAATGGAAAGACCTACTCTCATGGAGAGTCCTGGCACCCAAATCTACGAGCATTTGGCATTGTGGAATGT
  GTACTATGCACTTGTAATGTCACCAAGCAAGAATGTAAGAAAATCCACTGCCCCAATCGATACCCCTGCA
  AGTATCCTCAAAAAATAGATGGAAAGTGCTGCAAGGTGTGCCCAGGTAAAAAGGCAAAAGGTGCATTGGC
  TGGAGGCCCTGCCTTTGGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031258
• Insert Size: 1002 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031258.3, NP_112548.2
• RefSeq Size: 4190 bp
• RefSeq ORF: 1002 bp
• Locus ID: 83453
• UniProt ID: Q920C1
• Cytogenetics: X F2
• Gene Summary: Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation (By similarity). Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and lacks a portion of the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.