Mmab (NM_029956) Mouse Untagged Clone

CAT#: MC207769

Mmab (untagged) - Mouse methylmalonic aciduria (cobalamin deficiency) type B homolog (human) (Mmab), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mmab
• Synonyms: 9130222L19Rik; ATR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207769 representing NM_029956
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGTGGCTCTTTGGGGGTCGCCTCGGCCTGCGCGGGCGTCTCAGCGCCTGCAGACTGCTGTGTC
  CCCGTTTCCAGAGCCGTGGCCCTCAGGGCGGGGAAGACGGGGACAGGCTGCAGCCGTCCTCGACGGCAGC
  CAAGATCCCCAAGATTTACACCAAGACGGGAGACAAAGGGTTTTCCAGCACCTTCACAGGAGAAAGGAGA
  CCTAAAGATGATCAAGTATTTGAAGCCGTGGGGACCACGGATGAGCTAAGCTCAGCTATTGGGTTTGCCA
  TGGAGTTGGTCACAGAAAAAGGCCACATGTTTGCCGAAGAGCTTCAGAAAATCCAGTGCATGCTACAGGA
  CGTCGGCTCCGCCCTGGCCACTCCACGTTCCTCAGCCAGGGAGGCTCACTTAAAGCACACGGCCTTCCAG
  GAAGGGCCTGTCCTGGAGCTGGAGCGGTGGATTGACAAGTACTCCAGCCAGCTCCCCCCGCTCAAGGCCT
  TCATTCTGCCTTCTGGAGGCAAGAGCAGCTCTGCCCTGCACTTCTGCCGCGCTGTATGCCGTCGTGCCGA
  GAGACGTGTGGTGCCTCTTGTCCAGATGGGAGAGACGGATGCAAACGTGGCGAAGTTCTTAAACAGGCTC
  AGTGATTACCTCTTCACTGTAGCCAGATATGCAGCCATGAAGGAGGGCAGCCAAGAGAAAATATACAAGA
  AACATGATGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029956
• Insert Size: 714 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029956.3, NP_084232.1
• RefSeq Size: 3021 bp
• RefSeq ORF: 714 bp
• Locus ID: 77697
• UniProt ID: Q9D273
• Cytogenetics: 5 F
• Gene Summary: Adenosyltransferase involved in intracellular vitamin B12 metabolism. Generates adenosylcobalamin (AdoCbl) and directly delivers the cofactor to MUT in a transfer taht is stimulated by ATP-binding to MMAB and gated by MMAA.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).