Bfar (NM_025653) Mouse Untagged Clone

CAT#: MC207588

3110001I22Rik (untagged) - Mouse RIKEN cDNA 3110001I22 gene (3110001I22Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bfar
• Synonyms: 3010001A07Rik; 3110001I22Rik; AI666707; AW107665; Bar; Rnf47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207588 representing NM_025653
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCATATCCCGGTCATCCTTGGTCACCGTCCAGGATCAGTGTACAGAAGAGACAAGATGTGGCCTA
  ACAGATCATATGATGAAGTACCTCAGAATGTCTCCAGTGATGAATCTACCCTGAGGAAAACAAGAGGTGA
  TCTTTCTGCAAGCCAACAGCATGAGTGCGGGGATGAGAAAGATGACTTTAGAAGAAACTGTTCCTCTTCT
  TCCCATTACTCAGAACAGCAGTCTCCCCATAAAGGGGCTTCTTGTTTTTCCAGAAAATCAGGTATAGATC
  AGAAGAACTCCCCACACAGCAGATCTGGATCCAGTGGAAGCCATTCTCCAAAACAAAGCAAACTGCATTC
  TTCCCATGGGTCAGAGCTTAGGAAGTCCGAGCATGCTCATTCCTCCTACAAACGGCACAGTGAAGAAAAG
  CCAGAAGGAGGTAAAAAGAGGGCTGGCCAGTCTTTGAAAACATCAAGAGACACACACCCAAAAAGTTCTT
  CAGCAGTTCCTTCGTCAGAAAGTTTAAACAAACTCAAAAGGCTTGATGAAAAGGGACGTCCTAAAGCTGC
  AAGCAGGCAAGCTGTAGAAAAGCCAATGATGGCAGATGAAAGTGACTTACCTAAAATTTCTGAGTTTGAA
  GTTGGATTCTCAGAACTTATGGATCAGCCACAAGAACCTGGGTCAAACAAAAAAGATCATACAGAATTCA
  TTGATGACCAAGTAACCAATCGCCGTAAAGCAATAGTGTCAAAGACCAAGGAAATTGAACAAGCCTACTA
  CCAAGACTGTGAGACCTTCGGAATGGTGGTGAAAATGCTGGTTGAAAAAGACGCTTCATTAGAAAGGCCC
  ATTCAGTTTGCGCTCCGGCAGAATTTACATGAACTAAGTGAGCGCTGTGTGGAGGAGCTCAGGCAGTTCA
  TTGCAGATTATGATGCTGCTGCTTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025653
• Insert Size: 939 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025653.2, NP_079929.1
• RefSeq Size: 1611 bp
• RefSeq ORF: 939 bp
• Locus ID: 67118
• Cytogenetics: 16 A1
• Gene Summary: Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has multiple differences compared to variant 1. The encoded isoform (3) is completely distinct from isoform 1.