Extl2 (NM_021388) Mouse Untagged Clone

CAT#: MC207542

Extl2 (untagged) - Mouse exostoses (multiple)-like 2 (Extl2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Extl2
• Synonyms: 3000001D04Rik; AW146439
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207542 representing NM_021388
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGGGGTTGCCACATCTGCAAACTTCCGGGAAGAGTCATGGGGATTCGAGTGCTCCGGTTCTCCC
  TGGTGGTCATCCTTGTGTTGCTGCTGGTAGCGGGGGCTTTGACCAACTTACTCCCCAACATCAAAGAAGA
  CAAGATGCTCACTCTGCGTAGGGAGATCAAATCCCCGAGCAAGTCGGCCCTGGATTCATTCACCCTCATC
  ATGCAGACATACAACAGAACAGACCTCTTACTAAGACTCTTAAATCATTACCAGGCAGTACCGAGTCTGC
  ACAAGGTGATCGTGGTGTGGAACAATGTGGGAGAGAAGGGCCCAGAGGAGTTGTGGAATTCTCTAGGGCC
  TCACCCGATCCCTGTCATCTTCAAACCACAAACAGCAAACAAAATGAGAAACCGACTCCAGGTCTTCCCT
  GAAGTGGAGACCAACGCGGTGTTGATGGTAGACGATGACACACTCATTAGTGCCCAGGACCTTGTTTTCG
  CTTTCTCAATTTGGCAGCAATTTCCTGATCAGATCATAGGATTTGTACCTAGAAAGCACGTCTCTACTTC
  CTCCGGGATCTACAGTTACGGAGGCTTTGAGCTGCAGACACCGGGGCCCGGGAATGGCGACCAGTACTCC
  ATGGTGCTCATCGGAGCCTCCTTCTTCAACAGCAAATACCTGGAACTCTTCCAGAAGCAGCCTGCAGCCG
  TCCACGCGCTGATCGATGAGACGCAGAACTGTGACGACATCGCTATGAATTTCCTCGTCACCAGGCACAC
  TGGCAAGCCTTCAGGGATATTCGTGAAACCCATAAACATGGTCAACTTGGAAAAAGAAACCAACGGGTAT
  TCCGGAATGTGGCACCGGGCGGAGCACTTTCTGCAGAGATCCTACTGTATAAATAAGCTCGTTAACATCT
  ACGACGGCATGCCCTTAAAGTACTCCAACATTATGATTTCCCAGTTTGGTTTTCCGTATGCCAACCATAA
  AAGTAAAATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021388
• Insert Size: 993 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021388.4, NP_067363.3
• RefSeq Size: 3012 bp
• RefSeq ORF: 993 bp
• Locus ID: 58193
• UniProt ID: Q9ES89
• Cytogenetics: 3 G1
• Gene Summary: Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform.