Traf1 (NM_009421) Mouse Untagged Clone

CAT#: MC207429

Traf1 (untagged) - Mouse TNF receptor-associated factor 1 (Traf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Traf1
• Synonyms: 4732496E14Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207429 representing NM_009421
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCAGCTCAGCCCCTGATGAAAACGAGTTTCAATTTGGTTGCCCCCCTGCTCCCTGCCAGGACC
  CATCGGAGCCCAGAGTTCTCTGCTGCACAGCCTGTCTCTCTGAGAACCTGAGAGATGATGAGGATCGGAT
  CTGTCCTAAATGCAGAGCAGACAACCTCCATCCTGTGAGCCCAGGAAGCCCTCTGACTCAGGAGAAGGTT
  CACTCTGATGTAGCTGAGGCTGAAATCATGTGCCCCTTTGCAGGTGTTGGCTGTTCCTTCAAGGGGAGCC
  CACAATCCATGCAGGAGCATGAGGCTACCTCCCAGTCCTCCCACCTGTACCTGCTGCTGGCGGTCTTAAA
  GGAGTGGAAATCCTCACCAGGCTCCAACCTAGGGTCTGCACCCATGGCACTGGAGCGGAACCTGTCAGAG
  CTGCAGCTTCAGGCAGCTGTGGAAGCGACAGGGGACCTGGAGGTAGACTGCTACCGGGCACCTTGCTGTG
  AGAGCCAGGAAGAACTGGCCCTGCAGCACTTGGTGAAGGAGAAGCTGCTGGCTCAGCTGGAGGAGAAGCT
  GCGTGTGTTTGCAAACATTGTTGCTGTCCTCAACAAGGAAGTGGAGGCTTCCCACCTGGCACTGGCCGCC
  TCCATCCACCAGAGCCAGTTGGACCGAGAGCACATCCTGAGCTTGGAGCAGAGGGTGGTGGAATTACAGC
  AAACCCTGGCTCAAAAAGACCAGGTCCTGGGCAAGCTTGAGCACAGTCTGCGACTCATGGAGGAGGCATC
  CTTTGATGGTACTTTCCTGTGGAAGATCACCAATGTCACCAAGCGGTGCCACGAGTCAGTGTGTGGCCGG
  ACTGTCAGCCTCTTCTCTCCAGCTTTCTACACTGCCAAGTATGGTTACAAGTTGTGCCTGCGCTTGTACC
  TGAACGGGGATGGCTCAGGCAAGAAGACCCACCTGTCCCTCTTCATCGTGATCATGAGAGGAGAATACGA
  TGCTCTCCTGCCCTGGCCTTTCAGGAACAAGGTCACCTTTATGCTACTTGACCAGAACAACCGAGAGCAT
  GCTATTGATGCCTTCCGGCCTGACCTGAGCTCAGCCTCCTTCCAGCGGCCACAGAGTGAGACCAACGTGG
  CCAGCGGCTGCCCGCTCTTCTTCCCCCTCAGCAAGCTGCAGTCACCCAAGCACGCCTACGTCAAAGATGA
  CACAATGTTCCTCAAATGCATTGTGGACACTAGTGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009421
• Insert Size: 1230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009421.4, NP_033447.2
• RefSeq Size: 3833 bp
• RefSeq ORF: 1230 bp
• Locus ID: 22029
• UniProt ID: P39428
• Cytogenetics: 2 B
• Gene Summary: Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.