Nfyc (NM_001048168) Mouse Untagged Clone

CAT#: MC207333

Nfyc (untagged) - Mouse nuclear transcription factor-Y gamma (Nfyc), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfyc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207333 representing NM_001048168
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACAGAAGGAGGGTTTGGCGGTACCAGCAGCAGTGATGCCCAGCAAAGCCTCCAGTCCTTCTGGC
  CCAGAGTCATGGAAGAAATCCGAAACTTAACAGTGAAAGATTTCCGAGTACAGGAACTCCCACTGGCTCG
  TATTAAGAAGATTATGAAACTGGATGAAGATGTGAAGATGATCAGTGCAGAAGCCCCTGTGCTGTTTGCT
  AAGGCAGCCCAGATTTTTATCACTGAGCTGACTCTTCGAGCCTGGATCCACACAGAGGATAACAAGCGTC
  GTACTCTTCAGAGGAATGATATTGCCATGGCAATTACAAAATTTGATCAGTTCGACTTTCTCATCGATAT
  TGTTCCAAGAGATGAACTGAAACCTCCAAAGCGCCAGGAGGAGGTACGCCAGTCTGTGACTCCTGCGGAG
  CCTGTCCAGTACTACTTCACGCTGGCTCAGCAGCCCACTGCTGTCCAGGTCCAGGGACAGCAGCAAGGCC
  AGCAGACCACCAGTTCTACGACCACCATCCAGCCTGGACAGATCATCATTGCACAGCCTCAGCAGGGTCA
  GACCACACCCGTGACCATGCAGGTTGGAGAAGGTCAGCAGGTGCAGATTGTACAGGCCCAACCTCAGGGT
  CAGGCCCAGCAGACCCAGAGTGGCACTGGACAGACCATGCAGGTGATGCAGCAGATCATTACCAACACAG
  GAGAGATCCAGCAGATCCCGGTGCAGCTGAATGCTGGCCAGCTGCAATATATCCGATTAGCCCAGCCTGT
  ATCAGGCACCCAAGTTGTCCAAGGACAGATCCAGACACTTGCTACCAATGCCCAACAGATCACACAGACA
  GAGGTCCAGCAAGGACAGCAGCAGTTCAGCCAGTTCACAGATGGACAGCAGCTGTACCAGATCCAGCAAG
  TCACCATGCCTGCAGGCCAAGACCTCGCCCAGCCCATGTTTATTCAGTCAGCCAATCAGCCCTCTGATGG
  GCAGACCCCCCAGGTGACTGGAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001048168
• Insert Size: 1008 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048168.2, NP_001041633.1
• RefSeq Size: 1917 bp
• RefSeq ORF: 1008 bp
• Locus ID: 18046
• UniProt ID: P70353
• Cytogenetics: 4 56.52 cM
• Gene Summary: Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 4, 5 and 6 encode the same isoform (1).