Gale (NM_178389) Mouse Untagged Clone

CAT#: MC205432

Gale (untagged) - Mouse galactose-4-epimerase, UDP (Gale), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gale
• Synonyms: 2310002A12Rik; AI323962
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027438
  CAGAAACTGGTAGTTAGCTAGTTAGACCCAGGGCTGATTCTGGAACCTCTCCTTAAACTAGGGCTCACCC CTACATCCTCCAAGATGAAGGTAGGGGTCGAGCATCCAGTTGGAGTGAGGCCCTGAGCAGGAAGCCTCGG CCACCTCTGAAACTCTGTCTTCTAAGCAGGCGCCATGGAAAAGGTGCTGGTCACAGGTGGGGCTGGCTAC ATCGGCAGCCACACGGTATTGGAGCTGCTGGAGGCAGGCTACTCACCTGTGGTCATTGACAACTTCCATA ACGCCATTCGTGGAGAAGACTCCATGCCTGAGAGCCTGCGCCGGGTCCAGGAGTTGACAGGCCGCTCTGT GGAGTTTGAGGAGATGGACATCTTGGACCAGGCAGCCCTACAGCACCTCTTTAAGAAGCACAGCTTTAAG GCCGTCATCCACTTTGCTGGCCTCAAGGCTGTGGGCGAGTCAGTGCAGAAGCCTCTGGACTACTATAGAG TTAACTTAACAGGGACCATCCAGCTTCTAGAGATCATGAGGGCCCACGGAGTGAAGAACCTGGTGTTCAG CAGCTCAGCCACCGTGTACGGGAACCCCCAGTACCTGCCTCTGGATGAGGCCCACCCCACGGGGGGCTGT ACCAACCCCTACGGCAAGTCCAAGTTCTTCATCGAGGAGATGATCCGGGACCTGTGCCGGGCAGACACGG CCTGGAACGCCGTGCTGCTTCGGTACTTCAATCCCATAGGCGCCCACGCCTCTGGGCGCATCGGTGAAGA TCCTCAGGGTATTCCCAACAACCTCATGCCCTACGTCTCCCAGGTGGCAATCGGGCGACGAGAGGCCCTG AATGTCTTTGGTGATGACTATGCTACAGAGGACGGGACAGGTGTGAGGGATTACATTCACGTGGTGGACC TGGCGAAGGGCCATATAGCAGCCTTGAAGAAGCTGAAGGAGCAATGTGGTTGCCGGACCTACAACCTGGG CACGGGCACAGGCTACTCTGTCCTGCAGATGGTCCAAGCCATGGAGAAAGCCTCAGGGAAGAAGATCCCG TACAAGGTGGTGGCACGGCGGGAAGGTGATGTGGCGGCCTGTTATGCCAACCCCAGCCTGGCCCATGAGG AGCTGGGCTGGACAGCAGCCCTGGGGCTGGACAGGATGTGTGAAGATCTGTGGCGCTGGCAGAAGCAGAA CCCTTCGGGCTTTGGGGCGCAGGCCTGAGGACCTCGTTACCATGGACCAGAAAAGAGCAGCTGCCTGCTT TCCAGTCTCCACAGGACCCTGTACCCTCACACTCTGGGGCCACCCTACCTCAAATGCCAGCTGTCTGCTC AGCCCTCCAGGCAGGAGACCTCTGGTTCCACTGACCAGAAAGAAGTCCAGGTCTTTCCAGCCTGTCTCCC CACCGAGTTCAGCAGCTCATGTGATCCCCAGAGCCTTGGGGATGGGGAGGCCAGGGGGTCTGGGACCATA GCCTCCCCCAGGCACTGATGATTAAGCTTTCCAAAGTATTTAAAATAAAGATGTTTTCTTATCGAGAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_178389
• Insert Size: 1044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027438, AAH27438
• RefSeq Size: 1539 bp
• RefSeq ORF: 1044 bp
• Locus ID: 74246
• UniProt ID: Q8R059
• Cytogenetics: 4 D3
• Gene Summary: Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.[UniProtKB/Swiss-Prot Function]