Gale (NM_178389) Mouse Tagged ORF Clone

CAT#: MG205246

Gale (tGFP-tagged) - Mouse galactose-4-epimerase, UDP (Gale)

Product Images

Circular map for MG205246

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gale
• Synonyms: 2310002A12Rik; AI323962
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205246 representing NM_178389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAGGTGCTGGTCACAGGTGGGGCTGGCTACATCGGCAGCCACACGGTATTGGAGCTGCTGGAGG
  CAGGCTACTCACCTGTGGTCATTGACAACTTCCATAACGCCATTCGTGGAGAAGACTCCATGCCTGAGAG
  CCTGCGCCGGGTCCAGGAGTTGACAGGCCGCTCTGTGGAGTTTGAGGAGATGGACATCTTGGACCAGGCA
  GCCCTACAGCACCTCTTTAAGAAGCACAGCTTTAAGGCCGTCATCCACTTTGCTGGCCTCAAGGCTGTGG
  GCGAGTCAGTGCAGAAGCCTCTGGACTACTATAGAGTTAACTTAACAGGGACCATCCAGCTTCTAGAGAT
  CATGAGGGCCCACGGAGTGAAGAACCTGGTGTTCAGCAGCTCAGCCACCGTGTACGGGAACCCCCAGTAC
  CTGCCTCTGGATGAGGCCCACCCCACGGGGGGCTGTACCAACCCCTACGGCAAGTCCAAGTTCTTCATCG
  AGGAGATGATCCGGGACCTGTGCCGGGCAGACACGGCCTGGAACGCCGTGCTGCTTCGGTACTTCAATCC
  CATAGGCGCCCACGCCTCTGGGCGCATCGGTGAAGATCCTCAGGGTATTCCCAACAACCTCATGCCCTAC
  GTCTCCCAGGTGGCAATCGGGCGACGAGAGGCCCTGAATGTCTTTGGTGATGACTATGCTACAGAGGACG
  GGACAGGTGTGAGGGATTACATTCACGTGGTGGACCTGGCGAAGGGCCATATAGCAGCCTTGAAGAAGCT
  GAAGGAGCAATGTGGTTGCCGGACCTACAACCTGGGCACGGGCACAGGCTACTCTGTCCTGCAGATGGTC
  CAAGCCATGGAGAAAGCCTCAGGGAAGAAGATCCCGTACAAGGTGGTGGCACGGCGGGAAGGTGATGTGG
  CGGCCTGTTATGCCAACCCCAGCCTGGCCCATGAGGAGCTGGGCTGGACAGCAGCCCTGGGGCTGGACAG
  GATGTGTGAAGATCTGTGGCGCTGGCAGAAGCAGAACCCTTCGGGCTTTGGGGCGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205246 representing NM_178389
  Red=Cloning site Green=Tags(s)
  
  MEKVLVTGGAGYIGSHTVLELLEAGYSPVVIDNFHNAIRGEDSMPESLRRVQELTGRSVEFEEMDILDQA
  ALQHLFKKHSFKAVIHFAGLKAVGESVQKPLDYYRVNLTGTIQLLEIMRAHGVKNLVFSSSATVYGNPQY
  LPLDEAHPTGGCTNPYGKSKFFIEEMIRDLCRADTAWNAVLLRYFNPIGAHASGRIGEDPQGIPNNLMPY
  VSQVAIGRREALNVFGDDYATEDGTGVRDYIHVVDLAKGHIAALKKLKEQCGCRTYNLGTGTGYSVLQMV
  QAMEKASGKKIPYKVVARREGDVAACYANPSLAHEELGWTAALGLDRMCEDLWRWQKQNPSGFGAQA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178389
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178389.3, NP_848476.1
• RefSeq Size: 1537 bp
• RefSeq ORF: 1044 bp
• Locus ID: 74246
• UniProt ID: Q8R059
• Cytogenetics: 4 D3
• Gene Summary: Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.[UniProtKB/Swiss-Prot Function]