Kcne2 (NM_134110) Mouse Untagged Clone

CAT#: MC204104

Kcne2 (untagged) - Mouse potassium voltage-gated channel, Isk-related subfamily, gene 2 (Kcne2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcne2
• Synonyms: 2200002I16Rik; AW048273; MiRP1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC022699
  GGATCACACAGTGTTTCAGAGAGATGGTGGTAGCTGTGCAGAGAGTGTGGGGATGGCAATGAGAGGCAGA TTGAACGCAAGGGGGAAGCATGGCCACATTAGCCAATTTGACCCAGACACTGGAGGATGCCTTCAAAAAG ATTTTTATTACTTATATGGACAGCTGGAGGAGGAACACGACAGCCGAGGAGCAGGCACTCCAGGCCAGAG TGGATGCCGAGAACTTCTACTACGTCATCCTGTACCTCATGGTGATGATCGGCATGTTCTCGTTCATCGT GGTGGCCATCCTGGTGAGCACGGTGAAGTCGAAGCGGCGAGAGCACTCCCAGCACCCGTACCACCAGTAC ATCGTGGAAGATTGGCAGGAAAAGTACAAAAGTCAGATCCTGCATCTGGAAGACTCCAAGGCCACCATCC ATGAGAACATGGGGGCGACGGGGTTCACAGTGTCACCCTGATTGATAAGGAATGGGGGCCCGTCTGGCTC AGACAGGGTGCTTCTGCTGCCTCGAGAGCCCCACTTGTGCTTGCTCAGAAGACAGCTAGTTCCTCTGTGC AGCCTGGCTGGCAGTTGACACAGTGACATTTCTATCTCAGTGGTTTAGGTGAGCCTGTTGGGGTGATACT TTATGCTAAGGATCTCTTTTTTCACATGTTATGCAATGTAACTGTTATTTTAAACAATTTCAATCATGAG AACAGAGGCAAACATGAAGCAAGGGGACGGGGCAGGAGCAGTGGGAATAAATAGGAAATAGAAAGCAGAG ATTTACAAAGTTTTCAACCTTCCTTCTCATGAAATATTAGTTTGGATGTAGCAGATTCAGTTTATGAGTA AGTCGCACGTGTTCAGAAGCTGGGGTTTAAATCTCTCTCGGGCTTGCTGAACTGACAGGAGAGCATTTAC TTACCGCTTTATGTATGGGCAGCGAGCCCGAGGTGGTCAATGGAGCGAAGTGCGGGTTATTTTGCATTCA TAGCTTTCCTGGTATTTAACTGAGTTGGACATCTGGACAGAACAAACACTGAAAGGACAAATAACCCGCA CCAGAAGCAGCAAATATTCAAGAGCCCCCAGTGCAACCAAACGCACTTCTAACTTTTTATAGAAACTTAA ACTCTAAAAGCTCTCTGGCTTGAAAAAGTATGGCATTTTTTCCTAAACATCATATTCTTTATTTAATTTT AGATCCGCTTTATAGCTTATTTTCACACATATTTGACAAGATGGAAGCTACAGAACACCCCACTAGATGT TTGATGCAAGCTAGACAGTGACTACTTAATCCTTTGCACCACAGTCAGGGTGCTGGAGAGATGTTGGTCA AAGGCTACAAATTTTAATGGGGCTGGGGATGTACACCCATAATCCAACCCTCAGGGGACTGAGGTGGGAC AGGCTAGCTGAGGTTCCAAGCCATTCTAGACCCATTGCACTGCAAGGTCAGTAACATGCCTTTTGGTCTT AAAAACTGATACCTGAGAGGATAGTAAGTATCCTTACCACAAAACAGTAGCTATGTGAGATGATACATTA GTCATTCCACAATGTGTATATGCTTCAAAACACCATGTTGTGTGTGTAATACAATGTAACCTGTCCTTAA GAAAATAAAATGAATATTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_134110
• Insert Size: 372 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022699, AAH22699
• RefSeq Size: 1664 bp
• RefSeq ORF: 372 bp
• Locus ID: 246133
• UniProt ID: Q9D808
• Cytogenetics: 16 C4
• Gene Summary: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with HCN1 and HCN2 and increase potassium current (By similarity). Interacts with KCNQ1; forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.