Ttll4 (NM_001014974) Mouse Untagged Clone

CAT#: MC203423

Ttll4 (untagged) - Mouse tubulin tyrosine ligase-like family, member 4 (Ttll4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttll4
• Synonyms: 4632407P03Rik; AI451681; mKIAA0173
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC085151
  CAGAAAGCGGCAGAGCGTGGGTCGCGTGGCGGAGCTTGGTTGCTAGGGACACCTGAGGTCGCCCGCTCGC CCAGCAGGCGCAGCGAAGAAGCGGCGCGGAGCCGGCGTCCAGTCGCGGCGAAGTAGGGTACCGTAGAATA CTGCAACTCTGAGGCCTCGCACTGAAAGTGATTAATTGCTGAGCCCTGGATGTGATGAACTGCTGACTGG TGCTTGGCAGGCACTCCTGTGAGGTGGTGGCTGCTGATGCAGCGTGGTTGGGAAGAGGAAGAGTGCCCTG GAAACTCGGGAGACTTCACACAAGCTGACGGATTCCAAGATGCAGCTGGTGCTAGAGGTGTGTGGCCCCT TACCTGAGCCAGGCCATGAGGCCATGTTGCCATGATGTGGGCCCCTCATGGCCTCAGCAGGAACAGAGCA CTACAGTATTGGCCTGCGCCGAGGAAACAGCTTCAAGCAGAGACATCCCTCAGGCACAGTGTCTGCTTCA CCATCTGAGAAACCCTCGGAGGTCAAAGTCTGGTCTCAGGCCCATCAGCAGGTGAAGCCAATCTGGAAAC TGGAAAAGAAGCACGTAGGCACACTGTCAGCAGGGCTGGGCACCAGTTTCTTGGGAGTCCCATCCCAGCC AGCCTATTTCCTTTGCCCTAGCACTTTATGTAGCTCTGGGACCACAGCAGTCATTGCAGGCCACAGCAAC CCCTGTTACCTGCAGTCCCTCCCTAACTTGTTCAGCAATACTCTGCTGTACCGCCGCACCAACGTGAGGC AAAAACCATACCAGCAACTGGAATCTTTCTGCTTGCGTTCCAGCCCTTCAGAGAAAAGATCTTTTTCTCT CCCTCAGAAGGGTCTTCCTGTCAGTGTCACTGCCAATAAAGCCACATCTTCTACAGTCTTCCCCATGGCC CAGCCCATGGCAACTTCACCCACTGATCCATACCTCTCACTGGCAGCAGCTGGGGAGAACCCTTCAAGGA AGAGCCTGGCCTCTGCTATCTCAGGGAAGATTGCATCTCCACTGTCCTATAAGCCCATGCTGAACAACAA CTCCTTCATGAGGCCAAATAGCACTAAAGTGCCCTTATCACAAGCCACAGATGGCCTGAAGCCAGTATCC TCACCCAAGATCCAGCCTGTCTCATGGCATCATTCAGGGGGTACTGGGGACTGTGTACCCCAACCTGGTG ACCATAAGGTGCCCCAAAACATTGCTACTGTCTTAGATGATGTCACTGCCCCTATCACCCCATCTATCCC TAGCACCCTCAACATATCCACAGCCAGTGTTACCTCTTCCCAGTGCAGTCAGAGTAACTTTAGGATGGAG GCACATCCCTGTGGCCTGGATGAAAACCCGGATTCCCAGAGTGCTACTAAAGAGGTACACTTCACTGAGG CTGTGAGGAAGTTGGCTGAAAAAGGCTTGGAGAAGATGCCACGACAAGGCTACCAGTTTGAACAGGCTTG TTTTGTGAACCCCAGCTTCCAGTGGGGTCTTCTCAACAGAAGCAGGCGGTGGAAACCTCTGATGGGCCAG CGGTTTCCTCAGGAGGATATTGGATTAGACAGTGCGATCCTTCCTGGTACTTCAGACACCTTGGGGTTGG ACAGCACAGTCTTCTGTACCAAGAGGATCAGCATTCACCTCCTTGCCTCACATGTCCATGGGCTCAATCC CAGCCCTGCCTGTGGGTCTGCAGTTGACCCCCAGGTACTTGGAGAAGACAGAGCTCCTGTTCCCCCTTCT TCCCTCCAGCCTCTTGGTGTAGCTGAAGTGGCCACTCGTCTTTCTTCTGTCCATCTTGACCAGCCTGGGA AGGAGCCTGAGGAAGCCAAGGACCTGAACTCTTGTACTAAGGGTGGTGGTTCAGCTACTGACCTCCAGCC AAACCAGGTGGAGCCTGAAGATACAGAAGATGAACTGGGAGATGGTTTGGAAGATAGCTGCAGCCATGAT GAGAATGAAGAAGAGGAAGGAGACTCAGAGTGCTCTTCTTTAAGTGTTGTCTCCCCCAGCGAGTCAGTGG CATTAATATCTCGGAACTGTGTGGATTTGATGTCCAAATCCCTTCCCAATCATGAGAAAGTTGTACGGCC AGCCCTCATCTACAGTCTCTTCCCCAATGTAACCCCTACCATCTATTTTGGGACTCGGGATGAAAGAGTG GAGAAACTTCCTTGGGAACAGAGGAGGCTGCTTCGGTGGAAGATGAGCACAGTGACCCCCAACATTGTCA AGCAGACCATCGGTCGGTCACACTTCAAAATCAGCAAGAGAAATGATGACTGGCTGGGCTGCTGGGGTCA CCACATGAAGTCTCCTGGTTTTCGATCCATTAGAGAGCATCAGAAGCTAAACCACTTCCCAGGCTCCTTC CAGATTGGGAGGAAGGACCGGCTGTGGCGGAACCTGTCTCGCATGCAAAGCCGTTTTGGCAAGAAGGAGT TCAGTTTCTTTCCTCAGTCCTTTATCCTGCCCCAGGATTCCAAGCTCTTGCGCAAAGCCTGGGAGAGCAG CAGCCGCCAGAAGTGGATTGTGAAGCCACCAGCATCTGCTCGAGGCATTGGCATTCAGGTCATTCACAAA TGGAGTCAGCTCCCCAAGCGCAGGCCCCTCCTGGTACAGAGGTATCTACACAAGCCCTACCTCATTAGTG GCAGCAAATTTGATCTTCGGATCTATGTTTACGTGACTTCTTATGATCCTCTACGGATTTACCTCTTTTC GGATGGACTCGTCCGTTTTGCCAGTTGCAAGTATTCCCCTTCCATGAAGAGTCTTAGCAACAAGTTCATG CACCTGACCAACTACAGTGTCAATAAAAAGAACACAGAATACCAGGCTAATGCTGATGAGACTGCATGCC AAGGGCACAAATGGGCATTGAAAGCTTTGTGGAACTATCTGAGCCAGAAAGGAATTAATAGTGATGCCAT CTGGGAGAAGATAAAGGATGTTGTAGTCAAAACCATCATCTCGTCAGAGCCCTATGTGACTAACCTACTG AAGTTATATGTGCGGCGCCCCTATAGCTGTCATGAGCTCTTTGGTTTTGACATCATGCTGGATGAGAACC TGAAACCCTGGGTCCTAGAAGTCAACATTTCTCCAAGCCTCCATTCCAACTCTCCTCTGGACATCAGCAT CAAAGGCCAGATGATCCGAGACCTTCTGAACCTGGCTGGCTTTGTTCTGCCCAACATGGAGGATATCATC TCTAGTTCCAGCAGCCCCAGCAGCTCCAGCGGCTCCAGCACCAGCTTGCCAAGCTCTCCTAGAGACAAAT GTCAAATGACTCCAGAGCACTTCACTGCACAGAAGATGAAGAAAGCCTATTACTTGACCCAGAAAATTCC TGACCAGGACTTCTATGCGTCCGTGCTGGACGTCTTGACACCAGATGATGTTCGGGTTTTGGTGGAGATG GAAGATGAGTTTTCTCGTCGTGGTCAATTTGAAAGAATTTTTCCTTCCCGAATCTCTTCTCGATATCTTC GGTTTTTTGAGCAGCCACGATATTTCAACATTCTCACCACCCAGTGGGAACAGAAGTACCATGGAAACAA GCTCAAAGGAGTAGATCTGCTTCGGAATTGGTGCTACAAAGGCTTCCACACGGGGATTGTCTCTGACTCA GCCCCACTGTGGTCTCTGCCAACATCGCTCATGACTACCTCAAAGGGTGATGGGACACCAAATTCCGCCA GCAAGTCACGAAAGAAGAGCGCCTCTGAGGGAACCACACTGTCCTCTGAAGACAGGTCAACACCCAAGTC TAAGAAGAGTCAAGCTGGCCTGTCTCCCATATCCAGGAAGACTTTGTCCTCAAGGAGCAATGAGAACACC AGCAAACAGTCCAAGCGCTCTACCCCGGGGTTACCTGTCCTCAAGTACTCTGGGCAGAGTTCCAGACTTT CTGCTGCTTCTGCTTCCCAGTCAGTCACTGACTCCCGCTTGACTGCTGTGAGCTCGTGACTGGTCCTGCA CAGGAGCACTCAGCTACCTCAAGGGAACCAGCCTGCTGACCAGCCTGAGCCTCTACCCCGCCTCCACTTT GCCCCTCATCAGAGTATTTTTGAAGTGGTTCTATTGTAGATATGGGCATCTGGAGGGCTGGAAGGGCGAT GGTGATGGGGAGAAGGTGAGGAAGGTTCGCCCTCTGTCACCTTACTGTCTGGCTAGCACCTCATTTCTCA GTAGAGAAGACAGTGACAGTCAAGCAGCTTTATAAAGCAGGGTTTGGTTTCTAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
• Restriction Sites: AscI-NotI
• ACCN: NM_001014974
• Insert Size: 3582 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC085151, AAH85151
• RefSeq Size: 4341 bp
• RefSeq ORF: 3582 bp
• Locus ID: 67534
• UniProt ID: Q80UG8
• Cytogenetics: 1 C4
• Gene Summary: Glutamylase which preferentially modifies beta-tubulin and non-tubulin proteins, such as NAP1L1, NAP1L4 and CGAS (PubMed:17499049, PubMed:21074048, PubMed:20530212). Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step (PubMed:17499049, PubMed:21074048). Involved in formation of short side-chains (PubMed:20530212). Mediates initiation of polyglutamylation of nucleosome assembly proteins NAP1L1 and NAP1L4 (PubMed:17499049). Also acts as a monoglutamylase: generates monoglutamylation of CGAS, leading to impair the nucleotidyltransferase activity of CGAS (PubMed:26829768).[UniProtKB/Swiss-Prot Function]