Ttll4 (NM_001014974) Mouse Tagged ORF Clone

CAT#: MG211784

Ttll4 (tGFP-tagged) - Mouse tubulin tyrosine ligase-like family, member 4 (Ttll4)

Product Images

Circular map for MG211784

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ttll4
• Synonyms: 4632407P03Rik; AI451681; mKIAA0173
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211784 representing NM_001014974
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAGCAGGAACAGAGCACTACAGTATTGGCCTGCGCCGAGGAAACAGCTTCAAGCAGAGACATC
  CCTCAGGCACAGTGTCTGCTTCACCATCTGAGAAACCCTCGGAGGTCAAAGTCTGGTCTCAGGCCCATCA
  GCAGGTGAAGCCAATCTGGAAACTGGAAAAGAAGCACGTAGGCACACTGTCAGCAGGGCTGGGCACCAGT
  TTCTTGGGAGTCCCATCCCAGCCAGCCTATTTCCTTTGCCCTAGCACTTTATGTAGCTCTGGGACCACAG
  CAGTCATTGCAGGCCACAGCAACCCCTGTTACCTGCAGTCCCTCCCTAACTTGTTCAGCAATACTCTGCT
  GTACCGCCGCACCAACGTGAGGCAAAAACCATACCAGCAACTGGAATCTTTCTGCTTGCGTTCCAGCCCT
  TCAGAGAAAAGATCTTTTTCTCTCCCTCAGAAGGGTCTTCCTGTCAGTGTCACTGCCAATAAAGCCACAT
  CTTCTACAGTCTTCCCCATGGCCCAGCCCATGGCAACTTCACCCACTGATCCATACCTCTCACTGGCAGC
  AGCTGGGGAGAACCCTTCAAGGAAGAGCCTGGCCTCTGCTATCTCAGGGAAGATTGCATCTCCACTGTCC
  TATAAGCCCATGCTGAACAACAACTCCTTCATGAGGCCAAATAGCACTAAAGTGCCCTTATCACAAGCCA
  CAGATGGCCTGAAGCCAGTATCCTCACCCAAGATCCAGCCTGTCTCATGGCATCATTCAGGGGGTACTGG
  GGACTGTGTACCCCAACCTGGTGACCATAAGGTGCCCCAAAACATTGCTACTGTCTTAGATGATGTCACT
  GCCCCTATCACCCCATCTATCCCTAGCACCCTCAACATATCCACAGCCAGTGTTACCTCTTCCCAGTGCA
  GTCAGAGTAACTTTAGGATGGAGGCACATCCCTGTGGCCTGGATGAAAACCCGGATTCCCAGAGTGCTAC
  TAAAGAGGTACACTTCACTGAGGCTGTGAGGAAGTTGGCTGAAAAAGGCTTGGAGAAGATGCCACGACAA
  GGCTACCAGTTTGAACAGGCTTGTTTTGTGAACCCCAGCTTCCAGTGGGGTCTTCTCAACAGAAGCAGGC
  GGTGGAAACCTCTGATGGGCCAGCGGTTTCCTCAGGAGGATATTGGATTAGACAGTGCGATCCTTCCTGG
  TACTTCAGACACCTTGGGGTTGGACAGCACAGTCTTCTGTACCAAGAGGATCAGCATTCACCTCCTTGCC
  TCACATGTCCATGGGCTCAATCCCAGCCCTGCCTGTGGGTCTGCAGTTGACCCCCAGGTACTTGGAGAAG
  ACAGAGCTCCTGTTCCCCCTTCTTCCCTCCAGCCTCTTGGTGTAGCTGAAGTGGCCACTCGTCTTTCTTC
  TGTCCATCTTGACCAGCCTGGGAAGGAGCCTGAGGAAGCCAAGGACCTGAACTCTTGTACTAAGGGTGGT
  GGTTCAGCTACTGACCTCCAGCCAAACCAGGTGGAGCCTGAAGATACAGAAGATGAACTGGGAGATGGTT
  TGGAAGATAGCTGCAGCCATGATGAGAATGAAGAAGAGGAAGGAGACTCAGAGTGCTCTTCTTTAAGTGT
  TGTCTCCCCCAGCGAGTCAGTGGCATTAATATCTCGGAACTGTGTGGATTTGATGTCCAAATCCCTTCCC
  AATCATGAGAAAGTTGTACGGCCAGCCCTCATCTACAGTCTCTTCCCCAATGTAACCCCTACCATCTATT
  TTGGGACTCGGGATGAAAGAGTGGAGAAACTTCCTTGGGAACAGAGGAGGCTGCTTCGGTGGAAGATGAG
  CACAGTGACCCCCAACATTGTCAAGCAGACCATCGGTCGGTCACACTTCAAAATCAGCAAGAGAAATGAT
  GACTGGCTGGGCTGCTGGGGTCACCACATGAAGTCTCCTGGTTTTCGATCCATTAGAGAGCATCAGAAGC
  TAAACCACTTCCCAGGCTCCTTCCAGATTGGGAGGAAGGACCGGCTGTGGCGGAACCTGTCTCGCATGCA
  AAGCCGTTTTGGCAAGAAGGAGTTCAGTTTCTTTCCTCAGTCCTTTATCCTGCCCCAGGATTCCAAGCTC
  TTGCGCAAAGCCTGGGAGAGCAGCAGCCGCCAGAAGTGGATTGTGAAGCCACCAGCATCTGCTCGAGGCA
  TTGGCATTCAGGTCATTCACAAATGGAGTCAGCTCCCCAAGCGCAGGCCCCTCCTGGTACAGAGGTATCT
  ACACAAGCCCTACCTCATTAGTGGCAGCAAATTTGATCTTCGGATCTATGTTTACGTGACTTCTTATGAT
  CCTCTACGGATTTACCTCTTTTCGGATGGACTCGTCCGTTTTGCCAGTTGCAAGTATTCCCCTTCCATGA
  AGAGTCTTAGCAACAAGTTCATGCACCTGACCAACTACAGTGTCAATAAAAAGAACACAGAATACCAGGC
  TAATGCTGATGAGACTGCATGCCAAGGGCACAAATGGGCATTGAAAGCTTTGTGGAACTATCTGAGCCAG
  AAAGGAATTAATAGTGATGCCATCTGGGAGAAGATAAAGGATGTTGTAGTCAAAACCATCATCTCGTCAG
  AGCCCTATGTGACTAACCTACTGAAGTTATATGTGCGGCGCCCCTATAGCTGTCATGAGCTCTTTGGTTT
  TGACATCATGCTGGATGAGAACCTGAAACCCTGGGTCCTAGAAGTCAACATTTCTCCAAGCCTCCATTCC
  AACTCTCCTCTGGACATCAGCATCAAAGGCCAGATGATCCGAGACCTTCTGAACCTGGCTGGCTTTGTTC
  TGCCCAACATGGAGGATATCATCTCTAGTTCCAGCAGCCCCAGCAGCTCCAGCGGCTCCAGCACCAGCTT
  GCCAAGCTCTCCTAGAGACAAATGTCAAATGACTCCAGAGCACTTCACTGCACAGAAGATGAAGAAAGCC
  TATTACTTGACCCAGAAAATTCCTGACCAGGACTTCTATGCGTCCGTGCTGGACGTCTTGACACCAGATG
  ATGTTCGGGTTTTGGTGGAGATGGAAGATGAGTTTTCTCGTCGTGGTCAATTTGAAAGAATTTTTCCTTC
  CCGAATCTCTTCTCGATATCTTCGGTTTTTTGAGCAGCCACGATATTTCAACATTCTCACCACCCAGTGG
  GAACAGAAGTACCATGGAAACAAGCTCAAAGGAGTAGATCTGCTTCGGAATTGGTGCTACAAAGGCTTCC
  ACACGGGGATTGTCTCTGACTCAGCCCCACTGTGGTCTCTGCCAACATCGCTCATGACTACCTCAAAGGG
  TGATGGGACACCAAATTCCGCCAGCAAGTCACGAAAGAAGAGCGCCTCTGAGGGAACCACACTGTCCTCT
  GAAGACAGGTCAACACCCAAGTCTAAGAAGAGTCAAGCTGGCCTGTCTCCCATATCCAGGAAGACTTTGT
  CCTCAAGGAGCAATGAGAACACCAGCAAACAGTCCAAGCGCTCTACCCCGGGGTTACCTGTCCTCAAGTA
  CTCTGGGCAGAGTTCCAGACTTTCTGCTGCTTCTGCTTCCCAGTCAGTCACTGACTCCCGCTTGACTGCT
  GTGAGCTCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211784 representing NM_001014974
  Red=Cloning site Green=Tags(s)
  
  MASAGTEHYSIGLRRGNSFKQRHPSGTVSASPSEKPSEVKVWSQAHQQVKPIWKLEKKHVGTLSAGLGTS
  FLGVPSQPAYFLCPSTLCSSGTTAVIAGHSNPCYLQSLPNLFSNTLLYRRTNVRQKPYQQLESFCLRSSP
  SEKRSFSLPQKGLPVSVTANKATSSTVFPMAQPMATSPTDPYLSLAAAGENPSRKSLASAISGKIASPLS
  YKPMLNNNSFMRPNSTKVPLSQATDGLKPVSSPKIQPVSWHHSGGTGDCVPQPGDHKVPQNIATVLDDVT
  APITPSIPSTLNISTASVTSSQCSQSNFRMEAHPCGLDENPDSQSATKEVHFTEAVRKLAEKGLEKMPRQ
  GYQFEQACFVNPSFQWGLLNRSRRWKPLMGQRFPQEDIGLDSAILPGTSDTLGLDSTVFCTKRISIHLLA
  SHVHGLNPSPACGSAVDPQVLGEDRAPVPPSSLQPLGVAEVATRLSSVHLDQPGKEPEEAKDLNSCTKGG
  GSATDLQPNQVEPEDTEDELGDGLEDSCSHDENEEEEGDSECSSLSVVSPSESVALISRNCVDLMSKSLP
  NHEKVVRPALIYSLFPNVTPTIYFGTRDERVEKLPWEQRRLLRWKMSTVTPNIVKQTIGRSHFKISKRND
  DWLGCWGHHMKSPGFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDSKL
  LRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYD
  PLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNTEYQANADETACQGHKWALKALWNYLSQ
  KGINSDAIWEKIKDVVVKTIISSEPYVTNLLKLYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHS
  NSPLDISIKGQMIRDLLNLAGFVLPNMEDIISSSSSPSSSSGSSTSLPSSPRDKCQMTPEHFTAQKMKKA
  YYLTQKIPDQDFYASVLDVLTPDDVRVLVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQW
  EQKYHGNKLKGVDLLRNWCYKGFHTGIVSDSAPLWSLPTSLMTTSKGDGTPNSASKSRKKSASEGTTLSS
  EDRSTPKSKKSQAGLSPISRKTLSSRSNENTSKQSKRSTPGLPVLKYSGQSSRLSAASASQSVTDSRLTA
  VSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014974
• ORF Size: 3579 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014974.2
• RefSeq Size: 4341 bp
• RefSeq ORF: 3582 bp
• Locus ID: 67534
• UniProt ID: Q80UG8
• Cytogenetics: 1 C4
• Gene Summary: Glutamylase which preferentially modifies beta-tubulin and non-tubulin proteins, such as NAP1L1, NAP1L4 and CGAS (PubMed:17499049, PubMed:21074048, PubMed:20530212). Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step (PubMed:17499049, PubMed:21074048). Involved in formation of short side-chains (PubMed:20530212). Mediates initiation of polyglutamylation of nucleosome assembly proteins NAP1L1 and NAP1L4 (PubMed:17499049). Also acts as a monoglutamylase: generates monoglutamylation of CGAS, leading to impair the nucleotidyltransferase activity of CGAS (PubMed:26829768).[UniProtKB/Swiss-Prot Function]