Caspase 3 (CASP3) Rabbit Monoclonal Antibody [Clone ID: R04-5C8]

CAT#: TA383877

Cleaved-Caspase 3 p17 Rabbit monoclonal Antibody

Product Images

Western blot detection of Caspase-3 in C6,3T3,Hela cell lysates using Caspase-3 Rabbit mAb(1:1000 diluted).Predicted band size:32kDa.Observed band size:32/17kDa.

Specifications

Product Data

• Clone Name: R04-5C8
• Applications: WB
• Recommended Dilution: WB: 1/1000
• Reactivities: Mouse, Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of mouse Caspase-3 (Cleaved)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 32 kDa; Observed MW: 32,17 kDa
• Gene Name: caspase 3
• Database Link:
  Entrez Gene 836 Human
  UniProt ID P42574
• Background: Swiss-Prot Acc.P70677.Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage . Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
• Synonyms: apopain; CASP-3; CPP-32; CPP32; CPP32B; procaspase3; SCA-1; Yama