IL20 Goat Polyclonal Antibody

CAT#: PP1209B2

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IL20 goat polyclonal antibody, Biotin



USD 399.00


Availability*
2 Weeks

Size
    • 50 ug


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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Human IL-20 by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~0.25-1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 2000-4000 pg/ml of recombinant Human IL-20.
Sandwich ELISA: To detect Human IL-20 by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. Used in conjunction with Purified anti-IL-20 Antibody (Cat.-No PP1209P1 or PP1209P2) as a Capture Antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human IL-20.
Western Blot: To detect Human IL-20 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Goat
Clonality Polyclonal
Immunogen Highly pure (>98%) E.coli derived, 35.2 kDa recombinant Human IL-20.
Formulation PBS, pH 7.2 without preservatives
Label: Biotin
State: Lyophilized purified Ig fraction
Label: conjugated
Reconstitution Method Restore to a concentration of 0.1-1.0 mg/ml with sterile PBS solution containing 0.1% BSA.
Purification Affinity Chromatography
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background IL20 is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.
Synonyms IL-20, ZCYTO10
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.