IL20 Goat Polyclonal Antibody

CAT#: PP1209B2

IL20 goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human IL-20 by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~0.25-1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 2000-4000 pg/ml of recombinant Human IL-20.
  Sandwich ELISA: To detect Human IL-20 by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. Used in conjunction with Purified anti-IL-20 Antibody (Cat.-No PP1209P1 or PP1209P2) as a Capture Antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human IL-20.
  Western Blot: To detect Human IL-20 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived, 35.2 kDa recombinant Human IL-20.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Label: conjugated
• Reconstitution Method: Restore to a concentration of 0.1-1.0 mg/ml with sterile PBS solution containing 0.1% BSA.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 20
• Database Link:
  Entrez Gene 50604 Human
  UniProt ID Q9NYY1
• Background: IL20 is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.
• Synonyms: IL-20, ZCYTO10