Claudin 5 (CLDN5) Mouse Monoclonal Antibody [Clone ID: OTI1G4]

CAT#: CF500843

Carrier-free (BSA/glycerol-free) CLDN5 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)

Product Images

Anti-CLDN5 mouse monoclonal antibody (TA500843) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CLDN5 (RC207122).

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  HEK293T cells transfected with either RC207122 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CLDN5 antibody (TA500843), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1G4
• Applications: FC, IF
• Recommended Dilution: IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CLDN5(NP_003268) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23 kDa
• Gene Name: claudin 5
• Database Link:
  NP_003268
  Entrez Gene 12741 MouseEntrez Gene 65131 RatEntrez Gene 7122 Human
  UniProt ID O00501
• Background: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: AWAL; BEC1; CPETRL1; TMVCF

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction