Collagen I (COL1A1) Rabbit Polyclonal Antibody

CAT#: BP8004

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Collagen I (COL1A1) rabbit polyclonal antibody, Purified



USD 1,050.00


Availability*
2 Weeks

Size
    • 500 ul


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Specifications

Product Data
Applications ELISA, IF, IHC, R
Recommended Dilution IF: 1:40
IHC(P): 1:200 - 1:600; 60 min at RT or 2-8°C over night.
ELISA.
RIA.
Positive control: goldfish skin.
Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Reactivity Fish, Goldfish
Host Rabbit
Clonality Polyclonal
Immunogen Purified collagen type I from goldfish skin
Specificity This antibody is specific for collagen type I.
Formulation Phosphate buffered solution; no BSA and preservative added
State: Purified
State: Lyophilized Ig fraction
Reconstitution Method Restore with 0.5 ml distilled water, for further dilution use appropriate antibody diluent.
Concentration lot specific
Purification Affinity chromatography
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens collagen type I alpha 1 chain (COL1A1)
Background Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
Synonyms COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data
Protein Families Druggable Genome
Protein Pathways ECM-receptor interaction, Focal adhesion
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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