Collagen I (COL1A1) Rabbit Polyclonal Antibody
Collagen I (COL1A1) rabbit polyclonal antibody, Purified
|Applications||ELISA, IF, IHC, R|
|Recommended Dilution||IF: 1:40
IHC(P): 1:200 - 1:600; 60 min at RT or 2-8°C over night.
Positive control: goldfish skin.
Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
|Immunogen||Purified collagen type I from goldfish skin|
|Specificity||This antibody is specific for collagen type I.|
|Formulation||Phosphate buffered solution; no BSA and preservative added
State: Lyophilized Ig fraction
|Reconstitution Method||Restore with 0.5 ml distilled water, for further dilution use appropriate antibody diluent.|
|Concentration||app. 1 mg/ml|
|Storage||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.|
|Stability||Shelf life: one year from despatch.|
|Gene Name||Homo sapiens collagen type I alpha 1 chain (COL1A1)|
|Background||Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
|Synonyms||COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen|
|Protein Families||Druggable Genome|
|Protein Pathways||ECM-receptor interaction, Focal adhesion|