Adeno-associated Virus / AAV (VP1 + VP2 + VP3) Mouse Monoclonal Antibody [Clone ID: B1]

CAT#: BM5015

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Adeno-associated Virus / AAV (VP1 + VP2 + VP3) mouse monoclonal antibody, clone B1, Purified



USD 767.00

2 Weeks*

Size
    • 1 ml

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Specifications

Product Data
Clone Name B1
Applications IF, IHC, IP, WB
Recommended Dilution Immunohistochemistry on Frozen Sections: assay dependent.
Immunohistochemistry on Paraffin Sections: assay dependent.
Western Blot: 1/250.
Immunoprecipitation: assay dependent;(mainly free VP proteins).
Affinity Chromatography.
Immunofluorescence Microscopy.
Reactivities Adenovirus 2
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen AAV2 capsids
Specificity Useful for Immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. BM5010).
B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm.
Epitope mapping experiments (Wobus et al., see below) identified amino acids 726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region.
The antibody is also useful for characterization of different stages of infection.
Reacts with AAV-2, found in Human and Monkey, and all published AAV serotypes (AAV-4 only weak cross-reaction, 11 &12 no cross-reaction).
Formulation Formulation: PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
State: Liquid purified IgG fraction
Concentration lot specific
Purification Affinity Chromatography on Protein A (≥ 95% pure by SDS-PAGE)
Conjugation Unconjugated
Storage Up to 1 month: 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles;
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Synonyms AAV1, AAV2, AAV3, AAV4, AAV5
Note Lyophilized to liquid change in 2021
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.