Adeno-associated Virus / AAV (VP1 + VP2 + VP3) Mouse Monoclonal Antibody [Clone ID: B1]
Adeno-associated Virus / AAV (VP1 + VP2 + VP3) mouse monoclonal antibody, clone B1, Purified
|Applications||IF, IHC, IP, WB|
|Recommended Dilution||Immunohistochemistry on Frozen Sections: assay dependent.
Immunohistochemistry on Paraffin Sections: assay dependent.
Western Blot: 1/250.
Immunoprecipitation: assay dependent;(mainly free VP proteins).
|Specificity||Useful for Immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. BM5010).
B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm.
Epitope mapping experiments (Wobus et al., see below) identified amino acids 726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region.
The antibody is also useful for characterization of different stages of infection.
Reacts with AAV-2, found in Human and Monkey, and all published AAV serotypes (AAV-4 only weak cross-reaction, 11 &12 no cross-reaction).
|Formulation||Formulation: PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
State: Liquid purified IgG fraction
|Purification||Affinity Chromatography on Protein A (≥ 95% pure by SDS-PAGE)|
|Storage||Up to 1 month: 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles;
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||AAV1, AAV2, AAV3, AAV4, AAV5|
|Note||Lyophilized to liquid change in 2021|