Adeno-Associated Virus 2 / AAV2 (intact particle) Mouse Monoclonal Antibody [Clone ID: A20]

CAT#: BM5010

Reviews ()
Write a review

Adeno-Associated Virus 2 / AAV2 (intact particle) mouse monoclonal antibody, clone A20, Purified



Get 29% off any Over-Expression Cell Lysate, a validated WB control, with any Ab purchase. Use code “OEL29“. View details.

USD 510.00


Availability*
2 Weeks

Size
    • 50 ug


Product images

Specifications

Product Data
Clone Name A20
Applications ELISA, FN, IF, IHC, IP
Recommended Dilution ELISA.
Immunoprecipitation: 1/5.
Neutralization Assay.
Affinity Chromatography.
Immunofluorescence Microscopy: 1/20 Overnight at 2-8°C.
Immunohistochemistry: Overnight at 2-8°C.
Reactivity Human, Monkey
Host Mouse
Isotype IgG3
Clonality Monoclonal
Immunogen Adeno-associated virus capsid proteins and virus particles.
Specificity For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. Clone A20 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.
The antibody cannot be used for Immunoblotting.
Epitope mapping experiments (Wobus et al., see below) identified four immunoreactive (discontinous) regions.
The major reaction was attributed to sequence aa 369 to 378 of AAV-2 capsids.
The antibody is also useful for Neutralizing experiments (cf. Moskalenko et.al).
Reacts with AAV-2, found in Human and Monkey. In ELISA also Applicable to AAV-3.
Formulation State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 1 ml distilled water (final solution contains 0.09% Sodium Azide, 0.5% BSA in PBS buffer, pH 7.4)
Purification Affinity Chromatography on Protein A
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. AAV can infect both dividing and non-dividing cells and may incorporate its genome into that of the host cell. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Serotype 2 (AAV2) has been the most extensively examined so far. AAV2 presents natural tropism towards skeletal muscles, neurons, vascular smooth muscle cells and hepatocytes.
Synonyms AAV-2
Reference Data
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Antibody Performance Guarantee banner
ANTIBODY VALIDATION 101
Clone ID reveals the Source of Monoclonal Antibodies