NeuN(RBFOX3) Chicken Polyclonal Polyclonal Antibody
Chicken Polyclonal Anti-RBFOX3 Antibody, Aff - PurifiedAntibody Service
Product Datasheet for 'AP31812PU-N'
|Recommend Dilution||IHC, ICC/IF|
|Immunogen||Synthetic peptide KLH conjugated corresponding to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the Human (NP_001076044) and Mouse (NP_001020102) sequences.
Production: After repeated injections int
|Formulation||10mM PBS, pH 7.2 containing 50% Glycerol and 0.02% Sodium Azide as preservative.|
|Reconstitution Method||Reconstitute with PBS (pH7.3). To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)|
|Concentration||0.1 mg/ml (based on Bradford assay readings using BSA as a standard).|
|Purification||Affinity Chromatography using a peptide column.|
|Predicted Protein Size||33.7KDa|
|Gene Name||RNA binding protein, fox-1 homolog 3|
|Background||Human Neu-N (Fox3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons, and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and Mouse antibodies against various cytoplasmic neuronal antigens.|
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