HuSH-29: Pre-designed shRNA

HuSH-29 are pre-designed shRNA with genome wide coverage (human and mouse). It takes advantage of the superiority of 29mer shRNA and delivers guaranteed knock-down. A new offering of pGFP-v-RS vector enables easy monitoring of transfection efficiency. (Learn more about HuSH-29...) The same superior design and construction for species other than human and mouse genes is offered through Exact-shRNA.

Features and Benefits

  • Two retroviral vector options: pRS vector and pGFP-V-RS vector
  • Easy monitoring of transfection with the pGFP-V-RS vector
  • 5ug of purified plasmid for immediate transfection
  • Guaranteed successful gene knockdown
  • Versatile applications: transient or stable transfection or retroviral infection
PGFP-V-RS Comparison
Left: HEK293T cells transfected with the pGFP-V-RS which express turboGFP. Right: HEK293T cells co-transfected with pGFP-V-RS and an shRNA constructs against turboGFP gene.
RNAi
Legend: Four individual shRNA constructs against HIF1A was transiently transfected into HEK293T cells. Three out of 4 constructs in kit TR320380 demonstrated effective knockdown in western blot using anti-HIF1A antibody. Erk1 and Erk2 was used as control.

Applications:

  • Gene silencing through transient or stable transfection
  • Gene silencing through retroviral transduction (see protocol)

Useful Links

Vector Application FAQ Citation Application Guide

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