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Human BNP (NPPB) ELISA Kit 1 x 96

Assay Type Sandwich
Sensitivity 6.99pg/ml
Sample Type Human serum, plasma and other biological fluids

qSTAR qPCR primer pairs against Homo sapiens gene NPPB

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

NPPB HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T

NPPB (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Natriuretic peptides B (27-134, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

Natriuretic peptides B (27-134, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

NPPB CRISPRa kit - CRISPR gene activation of human natriuretic peptide B

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

qPCR primer pairs and template standards against Homo sapiens gene NPPB

Application Plasmid of exact quantity for transcript copy number calculation

NPPB - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

NPPB - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

NPPB - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin

USD 1,174.00

4 Weeks

Transient overexpression of NPPB in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

Other Names BNP; Iso-ANP
Accession Number NM_002521, NP_002512