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BCAM (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

BCAM HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T

BCAM HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T

For quantitative detection of human BCAM in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Assay Type Sandwich ELISA kit of Quantitative Detection for human BCAM
Format 8x12 divisible strips
Reactivities Human

BCAM CRISPRa kit - CRISPR gene activation of human basal cell adhesion molecule (Lutheran blood group)

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

qSTAR qPCR primer pairs against Homo sapiens gene BCAM

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

3' UTR clone of basal cell adhesion molecule (Lutheran blood group) (BCAM) transcript variant 1 for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

3' UTR clone of basal cell adhesion molecule (Lutheran blood group) (BCAM) transcript variant 2 for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

BCAM (untagged)-Human basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 2

Vector pCMV6 series
Tag Tag Free

BCAM - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

BCAM - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

BCAM - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin