FLT3 Human Recombinant Protein

CAT#: TP723930

Human FLT3 Protein, hFc-His Tag

Product Images

Figure 1. Human FLT3, hFc-His Tag on SDS-PAGE under reducing condition.

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  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human FLT3LG, mFc-His tagged protein (TP723956) can bind Human FLT3, hFc-His tagged protein (TP723930) in a linear range of 0.128-10.02 ng/ml.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  FLT3（Asn27-Ser543）+hFc（Glu99-Ala330）+6×His tag
• Tag: C-Human Fc and 6×His
• Predicted MW: 125-130 kDa
• Purity: > 95%
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
• Reconstitution: Reconstitute with deionized water
• Preparation: Affinity purification
• Applications: ELISA
• Storage: Store the lyophilized protein at -20°C.
  After reconstitution, store the protein at -80°C for 12 months.
  Avoid repeated freezing and thawing.
• Stability: 12 months from date of despatch

Reference Data

• Locus ID: 2322
• UniProt ID: P36888
• Summary: Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.