ROR1 (NM_005012) Human Recombinant Protein

CAT#: TP314967L

Recombinant protein of human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1, 1 mg

Product Images

Serum concentrations (ng/ml) of anti-ROR1 autoantibodies in chronic lymphocytic leukemia (CLL) patients and controls, as measured by ELISA against the full-length ROR1 protein (OriGene TP314967). Solid circles: non-progressive CLL; crosses: progressive CLL; blank circles: control donors. Dotted line represents mean+2SD of controls. *** p < 0.0001. Figure cited from PLoS ONE, PMID: 26562161

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  Anti-ROR1 autoantibodies from three non-progressive (NP) and three progressive (P) chronic lymphocytic leukemia patients were analyzed in Western blot using the recombinant full-length ROR1 protein (OriGene TP314967). A goat anti-ROR1 antibody and an isotype control MAb served as positive and negative controls, respectively. Figure cited from PLoS ONE, PMID: 26562161

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  Cytotoxicity (%) of leukemic cells induced by the serum IgG fractions from five non-progressive (circles) and five progressive (crosses) chronic lymphocytic leukemia patients before and after the adsorption of sera with the recombinant full-length ROR1 protein (OriGene TP314967). *** p < 0.0001. Figure cited from PLoS ONE, PMID: 26562161

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  Coomassie blue staining of purified ROR1 protein (Cat# TP314967). The protein was produced from HEK293T cells transfected with ROR1 cDNA clone (Cat# RC214967) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC214967 representing NM_005012
  Red=Cloning site Green=Tags(s)
  
  MHRPRRRGTRPPLLALLAALLLAARGAAAQETELSVSAELVPTSSWNISSELNKDSYLTLDEPMNNITTS
  LGQTAELHCKVSGNPPPTIRWFKNDAPVVQEPRRLSFRSTIYGSRLRIRNLDTTDTGYFQCVATNGKEVV
  SSTGVLFVKFGPPPTASPGYSDEYEEDGFCQPYRGIACARFIGNRTVYMESLHMQGEIENQITAAFTMIG
  TSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEILENVLCQTEYIFARSNPMILMRLKLP
  NCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHTFTAL
  RFPELNGGHSYCRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEKNKMEILYILVPSVAIPLAIAL
  LFFFICVCRNNQKSSSAPVQRQPKHVRGQNVEMSMLNAYKPKSKAKELPLSAVRFMEELGECAFGKIYKG
  HLYLPGMDHAQLVAIKTLKDYNNPQQWMEFQQEASLMAELHHPNIVCLLGAVTQEQPVCMLFEYINQGDL
  HEFLIMRSPHSDVGCSSDEDGTVKSSLDHGDFLHIAIQIAAGMEYLSSHFFVHKDLAARNILIGEQLHVK
  ISDLGLSREIYSADYYRVQSKSLLPIRWMPPEAIMYGKFSSDSDIWSFGVVLWEIFSFGLQPYYGFSNQE
  VIEMVRKRQLLPCSEDCPPRMYSLMTECWNEIPSRRPRFKDIHVRLRSWEGLSSHTSSTTPSGGNATTQT
  TSLSASPVSNLSNPRYPNYMFPSQGITPQGQIAGFIGPPIPQNQRFIPINGYPIPPGYAAFPAAHYQPTG
  PPRVIQHCPPPKSRSPSSASGSTSTGHVTSLPSSGSNQEANIPLLPHMSIPNHPGGMGITVFGNKSQKPY
  KIDSKQASLLGDANIHGHTESMISAEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 104.1 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Bioactivity: ELISA capture for autoantibodies (PMID: 26562161)
  WB positive control (PMID: 26562161)
  Affinity purification chromatography (PMID: 26562161)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_005003
• Locus ID: 4919
• UniProt ID: Q01973
• Cytogenetics: 1p31.3
• Refseq Size: 3358
• Refseq ORF: 2811
• Synonyms: dJ537F10.1; NTRKR1
• Summary: This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane