Estrogen Receptor 1 (ESR1) Human Gene Knockout Kit (CRISPR)

CAT#: KN213277BN

ESR1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: Estrogen Receptor 1
• Locus ID: 2099
• Components:

  KN213277G1, Estrogen Receptor 1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN213277G2, Estrogen Receptor 1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN213277BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000125, NM_001122740, NM_001122741, NM_001122742, NM_001291230, NM_001291241, NM_001328100
• UniProt ID: P03372
• Synonyms: ER; Era; ESR; ESRA; ESTRR; NR3A1
• Summary: This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain. [provided by RefSeq, Jul 2020]