BCMA (TNFRSF17) Human Gene Knockout Kit (CRISPR)

CAT#: KN208851LP

BCMA/TNFRSF17 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: BCMA
• Locus ID: 608
• Components:

  KN208851G1, BCMA gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TAACGCTGACATGTTAGAGG

  KN208851G2, BCMA gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTGCTTGAACGATTATTCAT

  KN208851LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  CCTTTTGCCT TTTGACTCCC AAAGTCCTGG GATTACAGGT GTGAACCCCT GCAGCTGGCC TCAATGTTAA GATCTTAAGG GGCACAGCAC AGACCTTGTC TTGTCTATCT CCCTGGCACC TCTCACAGAG TACAGCTTAT TAACAGATGT TCCAGAAACT GTTACTGAAC ATTCGGCTTG ATGCTGTGGG CTTGTCTGCA TCTTGCAACT GTCACCTGGC TGAGAAATTT CTTCTATAAA TAAGCAGTTT CTGTTTCAGA TGTGATATGC CCTGATATTT ACACCCTGTC TCTTACCCCA TCCAAGACTC AAACTTAGAA ACTTGAATTA GATGTGGTAT TCAAATCCTT AGCTGCCGCG AAGACACAGA CAGCCCCCGT AAGAACCCAC GAAGCAGGCG AAGTTCATTG TTCTCAACAT TCTAGCTGCT CTTGCTGCAT TTGCTCTGGA ATTCTTGTAG AGATATTACT TGTCCTTCCA GGCTGTTCTT TCTGTAGCTC CCTTGTTTTC TTTTTGTGAT CATGTTGCAG ATGGCTGGGC AGTGCTCCCA AAATGAATAT TTTGACAGTT TGTTGCATGC TTGCATACCT TGTCAACTTC ATATGGACTG CTTATTCAGA GAATCAACAT AATGGGCATG ATGGTGAGTT TTCTTGAATC AAAAAGAGAA AGGAAGCAAG GCAGTGATTT TAATGTTTAT GGAAACAAAG TAATTATTTG GAACTGAACT TGATATGATT CAGCACTATT AGCAACATAG ATTTTTTTTA AAAATCAGCT CTTCTAATTA AGTGATATTT AGAATTTAAA AGTCAATGTT CATTAATTAA GGTGATTGAA TGGAAATAAT CCATACTTGA TTATTTTGCT ATCAAAACAA TCCATAATTC ATTATTTTAG CAAAATAATC AAGTATGACA GCCGGGTGCG GTGGATGGCT CACACCTGTA ATCCCAGCAC TTTGGGAGGC CGAGGTGGGT GAATCACCTG AGGTCGGCAG TTTGAGGCCA GCCTGGCCAA CCTGGTGAAA CCCTGTCTCT ACTAAAAATA CAAAAAATTA GCTGGGCATG GTGGCACAGG TCTGTAATCC CAGCTACTCG GGAGGCTGAG GCAGGAGAAT TGTTTGAACT TGGGAGGTGG AGGTTGCAGT GAGCCGAGAT CGCGCCACTG CACTCTAGCC TGGGCAACAG AGCAAGACTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001192
• UniProt ID: Q02223
• Synonyms: BCM; BCMA; CD269; TNFRSF13A
• Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq, Jul 2008]