Mouse Aifm1 activation kit by CRISPRa

CAT#: GA205043

Aifm1 CRISPRa kit - CRISPR gene activation of mouse apoptosis-inducing factor, mitochondrion-associated 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Aifm1
• Locus ID: 26926
• Kit Components:

  GA205043G1, Aifm1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA205043G2, Aifm1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA205043G3, Aifm1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001290364, NM_012019
• UniProt ID: Q9Z0X1
• Synonyms: AIF; AIFsh2; Hq; Pdcd8
• Summary: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity).[UniProtKB/Swiss-Prot Function]