Mouse Hif1a activation kit by CRISPRa

CAT#: GA201899

Hif1a CRISPRa kit - CRISPR gene activation of mouse hypoxia inducible factor 1, alpha subunit

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Hif1a
• Locus ID: 15251
• Kit Components:

  GA201899G1, Hif1a gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA201899G2, Hif1a gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA201899G3, Hif1a gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_010431
• UniProt ID: Q61221
• Synonyms: AA959795; bHLHe78; HIF-1-alpha; HIF1-alpha; HIF1alpha; MOP1
• Summary: This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]